Elimination kinetics of eugenol in grass carp in a simulated transportation setting

BMC Veterinary Research

Research Article

Xu-Feng Wang¹  Qi Liu¹  Dong-Hao Zhao¹  Chang-Liang Ke¹  Liu-Dong Li¹  Qiang Wang¹ 

[1]Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, 510300, Guangzhou, People’s Republic of China

[2]Laboratiry of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation, 510300, Guangzhou, People’s Republic of China

[3]Fishery Environments and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture, 510300, Guangzhou, People’s Republic of China

[4]South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People’s Republic of China

关键词: Ctenopharyngodon idella;    Clove oil;    Anesthetic;    Food safety risk;    Drug elimination;   

DOI  :  10.1186/s12917-017-1273-3

 received in 2017-09-06, accepted in 2017-11-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFish are vulnerable to stress from over-crowding during transportation and eugenol is the most common sedative used to minimize fish injury. The ADI value of 2.5 mg/kg is recommended by the Joint FAO/WHO Expert Committee on Food Additives. The aim of this work was to study the elimination kinetics of eugenol following exposure of grass carp to a eugenol bath in a simulated transportation setting.ResultsGrass carp, Ctenopharyngodon idella (120 fish) were exposed for 24 h to a 10 mg/L eugenol bath. Sampling was performed during a 96 h period after the 24 h bath. Eight fish were sampled at each time point and muscle, plasma and liver concentrations of the drug were determined by ultra-performance liquid chromatography-tandem mass spectrometry. The concentration-time data of eugenol in each tissue were analyzed using non-compartmental methods. The peak concentrations (Cmax) in plasma, muscle and liver were 7.68, 5.30 and 24.63 mg/kg and the elimination half-lives (t1/2β) were 19.79, 10.27 and 55.28 h, respectively. The clearance (CL) values were 0.10, 0.44 and 0.04 L/h/kg and the areas under the concentration-time curve (AUC0-96h) were 91.54, 22.44, and 214.12 mg·h/L in plasma, muscle and liver, respectively. After a eugenol exposure bath, drug concentrations in muscle tissue of grass carp were below 1 mg/kg at 8 h and 0.1 mg/kg at 24 h.ConclusionsThe drug concentrations in muscle tissue at 8 h were lower than the recommended ADI value.

【 授权许可】

CC BY   
© The Author(s). 2017