Analysis of antibody profiles in symptomatic malaria in three sentinel sites of Ivory Coast by using multiplex, fluorescent, magnetic, bead-based serological assay (MAGPIX™)

Malaria Journal

Research

Somela Brou¹  Laeticia Gnamien¹  Marie-France Ehouman¹  Joseph Allico Djaman¹  Sylvain Béourou²  André Offianan Touré²  David Koffi³  Vincent Richard⁴  Inès Vigan-Womas⁵  Ronald Perraut⁶  Marie-Louise Varela⁶ 

[1] UFR Biosciences, Université Félix Houphouet Boigny, Abidjan, Côte d’Ivoire;Unité de Paludologie, Institut Pasteur de Côte d’Ivoire, Abidjan, Côte d’Ivoire;Unité de Paludologie, Institut Pasteur de Côte d’Ivoire, Abidjan, Côte d’Ivoire;UFR Biosciences, Université Félix Houphouet Boigny, Abidjan, Côte d’Ivoire;Unité d’Epidémiologie, Institut Pasteur de Dakar, Dakar, Sénégal;Unité d’Immunologie des Maladies Infectieuses, Institut Pasteur de Madagascar, Antananarivo, Madagascar;Unité d’Immunologie, Institut Pasteur de Dakar, Dakar, Sénégal;

关键词: Malaria;    Plasmodium falciparum;    ELISA;    IgG;    Surface antigens;    Multiplex;    MAGPIX;    Ivory Coast;    Symptomatic malaria;    Biomarkers;   

DOI  :  10.1186/s12936-015-1043-2

 received in 2015-10-20, accepted in 2015-12-09,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAdvances in malaria control have reduced the burden of disease resulting from exposure to parasite infections. The consequences on naturally acquired immunity are unclear. A magnetic bead-based immunoassay (MBA) to assess antibody levels in populations living in endemic areas was previously evaluated. In this study, the effect of clinical attacks on immunity was analysed in three sentinel sites of Ivory Coast.MethodsRecombinant proteins or peptides derived from liver or blood stage antigens of Plasmodiumfalciparum (CSP, LSA141, LSA3, SALSA, PF13-DBL1α1, GLURP, AMA1, MSP1p19, MSP4p20), the CSP of Plasmodium malariae and the salivary glands antigen of Anopheles gambiae (gSG6) were covalently linked to a colour-coded microsphere (Luminex™ beads) for the multiplex assay. ELISA was used for whole parasite extract antigen. Blood samples (n = 94) of patients consulting for symptomatic malaria attacks and living in three different malaria endemic settings (rural and periurban) were analysed.ResultsHighly variable seroprevalence of antibody responses against parasite antigens was found ranging from 3 (gSG6) to 97 % (MSP4p20). A marked prevalence and significantly higher level of antibodies was found in patients from the rural site (Korhogo), those harbouring the lowest level of parasitaemia. The use of whole schizont extract could not discriminate immunity level, contrary to parasite-derived recombinant proteins or peptides. Prevalence of responders to LSA141 and levels of antibodies to PF13 were significantly different between the three settings. Moreover, the post-treatment clearance of parasites was clearly associated with a significantly higher level of antibody response for almost 50 % of the parasite antigens tested.ConclusionThe multiplex MBA-Magpix technology assay provides an accurate high throughput monitoring of parasite-specific antibodies during symptomatic malaria. The levels of antibody responses may provide a risk criterion with respect to the degree of parasitic infection. Additionally, they can be used as an indicator in the implementation of malaria prevention and local control strategies.

【 授权许可】

CC BY   
© Koffi et al. 2015