Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups

Annals of Clinical Microbiology and Antimicrobials

Short Report

Silvio Alencar Marques¹  Claudia Lima Witzel²  Maria de Lourdes Ribeiro de Souza da Cunha²  Camila Sena Martins de Souza²  Mariana Fávero Bonesso²  Carlos Magno Castelo Branco Fortaleza³  Mônica Silveira³ 

[1] Department of Dermatology and Radiology, Botucatu School of Medicine, University Hospital, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil;Department of Microbiology and Immunology, Botucatu Biosciences Institute, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil;Department of Tropical Diseases, Botucatu School of Medicine, University Hospital, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil;Department of Tropical Diseases, Botucatu School of Medicine, University Hospital, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil;

关键词: Staphylococcus aureus;    MSSA;    Toxins;    Cytotoxins;   

DOI  :  10.1186/s12941-016-0125-5

 received in 2015-09-10, accepted in 2016-02-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus is characterized by its pathogenicity and high prevalence, causing disease in both healthy and immunocompromised individuals due to its easy dissemination. This fact is aggravated by the widespread dissemination of S. aureus carrying toxigenic genes. The objective of this study was to determine the toxigenic profile of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in patients with purulent skin and/or soft tissue infections seen at the Dermatology Department of the University Hospital of the Botucatu Medical School, asymptomatic adults older than 60 years living in nursing homes, and prison inmates of the Avaré Detention Center.MethodsPCR was used for the detection of the mecA gene, enterotoxin genes (sea, seb, and sec), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin 1 (tst), panton–valentine leukocidin (lukS-PV and lukF-PV), and alpha- and delta-hemolysins or cytotoxins (hla and hld).ResultsThe results showed a significant prevalence of toxigenic genes among S. aureus isolates from asymptomatic individuals, with the observation of a higher prevalence of cytotoxin genes. However, the panton–valentine leukocidin gene was only detected in MSSA isolated from patients with skin infections and the tst gene was exclusively found in MSSA isolated from prison inmates.ConclusionsThe present study demonstrated a significant prevalence of toxigenic genes in MSSA and MRSA strains isolated from asymptomatic S. aureus carriers. There was a higher prevalence of cytotoxin genes.

【 授权许可】

CC BY   
© de Souza et al. 2016