Carbohydrate-active enzymes from pigmented Bacilli: a genomic approach to assess carbohydrate utilization and degradation

BMC Microbiology

Research Article

Bernard Henrissat¹  Pedro M Coutinho¹  Ezio Ricca²  Nicola Manzo²  Enrica D'Apuzzo²  Simon M Cutting³ 

[1] Architecture et Fonction des Macromolécules Biologiques, UMR6098, CNRS and Universités d'Aix-Marseille I & II, Case 932, 163 Av de Luminy, 13288, Marseille cedex 9, France;Department of Structural and Functional Biology, Federico II University of Naples, MSA, via Cinthia 4, 80126, Napoli, Italy;School of Biological Sciences, Royal Holloway University of London, TW20 0EX, Egham, Surrey, UK;

关键词: Carotenoid;    Glycoside Hydrolase;    Fructan;    Carbohydrate Binding Module;    Carotenoid Production;   

DOI  :  10.1186/1471-2180-11-198

 received in 2011-07-04, accepted in 2011-09-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSpore-forming Bacilli are Gram-positive bacteria commonly found in a variety of natural habitats, including soil, water and the gastro-intestinal (GI)-tract of animals. Isolates of various Bacillus species produce pigments, mostly carotenoids, with a putative protective role against UV irradiation and oxygen-reactive forms.ResultsWe report the annotation of carbohydrate active enzymes (CAZymes) of two pigmented Bacilli isolated from the human GI-tract and belonging to the Bacillus indicus and B. firmus species. A high number of glycoside hydrolases (GHs) and carbohydrate binding modules (CBMs) were found in both isolates. A detailed analysis of CAZyme families, was performed and supported by growth data. Carbohydrates able to support growth as the sole carbon source negatively effected carotenoid formation in rich medium, suggesting that a catabolite repression-like mechanism controls carotenoid biosynthesis in both Bacilli. Experimental results on biofilm formation confirmed genomic data on the potentials of B. indicus HU36 to produce a levan-based biofilm, while mucin-binding and -degradation experiments supported genomic data suggesting the ability of both Bacilli to degrade mammalian glycans.ConclusionsCAZy analyses of the genomes of the two pigmented Bacilli, compared to other Bacillus species and validated by experimental data on carbohydrate utilization, biofilm formation and mucin degradation, suggests that the two pigmented Bacilli are adapted to the intestinal environment and are suited to grow in and colonize the human gut.

【 授权许可】

Unknown   
© Manzo et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.