Selective inhibition of PfA-M1, over PfA-M17, by an amino-benzosuberone derivative blocks malaria parasites development in vitro and in vivo

Malaria Journal

Research

Marjorie Schmitt¹  Sébastien Albrecht²  Céline Tarnus²  Lotfi Bounaadja³  Elisabeth Mouray³  Isabelle Florent³ 

[1] Laboratoire de Chimie Moléculaire, CNRS-UMR7509, Université de Strasbourg, 67037, Strasbourg Cedex 2, France;Laboratoire de Chimie Organique et Bioorganique, EA4566, Université de Haute Alsace, 68093, Mulhouse Cedex, France;Molécules de Communication et Adaptation des Microorganismes, (MCAM, UMR7245), Muséum National Histoire Naturelle, Sorbonne Universités, CNRS, CP 52, 57 Rue Cuvier, 75005, Paris, France;

关键词: Malaria;    Plasmodium falciparum;    M1 aminopeptidase;    Chemotherapy;    Amino-benzosuberone derivative;   

DOI  :  10.1186/s12936-017-2032-4

 received in 2017-06-01, accepted in 2017-09-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPlasmodium falciparum M1 family aminopeptidase is currently considered as a promising target for anti-malarial chemotherapy. Several series of inhibitors developed by various research groups display IC50/Ki values down to nM range on native PfA-M1 or recombinant forms and block the parasite development in culture at µM to sub-µM concentrations. A handful of these inhibitors has been tested on murine models of malaria and has shown anti plasmodial in vivo activity. However, most of these inhibitors do also target the other neutral malarial aminopeptidase, PfA-M17, often with lower Ki values, which questions the relative involvement and importance of each enzyme in the parasite biology.ResultsAn amino-benzosuberone derivative from a previously published collection of chemicals targeting specifically the M1-aminopeptidases has been identified; it is highly potent on PfA-M1 (Ki = 50 nM) and devoid of inhibitory activity on PfA-M17 (no inhibition up to 100 µM). This amino-benzosuberone derivative (T5) inhibits, in the µM range, the in vitro growth of two P. falciparum strains, 3D7 and FcB1, respectively chloroquino-sensitive and resistant. Evaluated in vivo, on the murine non-lethal model of malaria Plasmodium chabaudi chabaudi, this amino-benzosuberone derivative was able to reduce the parasite burden by 44 and 40% in a typical 4-day Peters assay at a daily dose of 12 and 24 mg/kg by intraperitoneal route of administration.ConclusionsThe evaluation of a highly selective inhibitor of PfA-M1, over PfA-M17, active on Plasmodium parasites in vitro and in vivo, highlights the relevance of PfA-M1 in the biological development of the parasite as well as in the list of promising anti-malarial targets to be considered in combination with current or future anti-malarial drugs.

【 授权许可】

CC BY   
© The Author(s) 2017