Genomic and transcriptomic comparison between Staphylococcus aureus strains associated with high and low within herd prevalence of intra-mammary infection

BMC Microbiology

Research Article

M. Luini¹  E. Capra²  B. Castiglioni²  P. Cremonesi²  A. Stella³  A. Pietrelli⁴  S. Puccio⁵ 

[1] Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia, Sezione di Lodi, via Einstein, 26900, Lodi, Italy;Istituto di Biologia e Biotecnologia Agraria, CNR, via Einstein, 26900, Lodi, Italy;Istituto di Biologia e Biotecnologia Agraria, CNR, via Einstein, 26900, Lodi, Italy;Parco Tecnologico Padano, Via Einstein, 26900, Lodi, Italy;Istituto di Tecnologie Biomediche, CNR, Via Fratelli Cervi 93, 20090, Segrate, Milano, Italy;Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Via Francesco Sforza 35, 20122, Milan, Italy;Istituto di Tecnologie Biomediche, CNR, Via Fratelli Cervi 93, 20090, Segrate, Milano, Italy;Scuola di Dottorato in Medicina Molecolare e Traslazionale, Università di Milano, 20009, Segrate, Milan, Italy;

关键词: Staphylococcus aureus;    Mastitis;    Virulence;    Genome;    Transcriptome;    Next generation sequencing;   

DOI  :  10.1186/s12866-017-0931-8

 received in 2016-04-27, accepted in 2017-01-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus (Staph. aureus) is one of the major pathogens causing mastitis in dairy ruminants worldwide. The chronic nature of Staph. aureus infection enhances the contagiousness risk and diffusion in herds. In order to identify the factors involved in intra-mammary infection (IMI) and diffusion in dairy cows, we investigated the molecular characteristics of two groups of Staph. aureus strains belonging to ST8 and ST398, differing in clinical properties, through comparison of whole genome and whole transcriptome sequencing.ResultsThe two groups of strains, one originated from high IMI prevalence herds and the other from low IMI prevalence herds, present a peculiar set of genes and polymorphisms related to phenotypic features, such as bacterial invasion of mammary epithelial cells and host adaptation. Transcriptomic analysis supports the high propensity of ST8 strain to chronicity of infection and to a higher potential cytotoxicity.ConclusionsOur data are consistent with the invasiveness and host adaptation feature for the strains GTB/ST8 associated to high within-herd prevalence of mastitis. Variation in genes coding for surface exposed proteins and those associated to virulence and defence could constitute good targets for further research.

【 授权许可】

CC BY   
© The Author(s). 2017