Human iPSC-derived glia models for the study of neuroinflammation

Journal of Neuroinflammation

Review

Emily Maguire¹  Nina Stöberl¹  Elisa Salis¹  Bethany Shaw¹  Hazel Hall-Roberts¹ 

[1] UK Dementia Research Institute (UK DRI), School of Medicine, Cardiff University, CF10 3AT, Cardiff, UK;

关键词: Induced pluripotent stem cells;    iPSC;    Neuroinflammation;    Microglia;    Astrocytes;    Monoculture;    Co-culture;    Neural organoids;    Xenotransplantation;    Cytokines;   

DOI  :  10.1186/s12974-023-02919-2

 received in 2023-06-07, accepted in 2023-10-02,  发布年份 2023

来源: Springer

【 摘 要 】

Neuroinflammation is a complex biological process that plays a significant role in various brain disorders. Microglia and astrocytes are the key cell types involved in inflammatory responses in the central nervous system. Neuroinflammation results in increased levels of secreted inflammatory factors, such as cytokines, chemokines, and reactive oxygen species. To model neuroinflammation in vitro, various human induced pluripotent stem cell (iPSC)-based models have been utilized, including monocultures, transfer of conditioned media between cell types, co-culturing multiple cell types, neural organoids, and xenotransplantation of cells into the mouse brain. To induce neuroinflammatory responses in vitro, several stimuli have been established that can induce responses in either microglia, astrocytes, or both. Here, we describe and critically evaluate the different types of iPSC models that can be used to study neuroinflammation and highlight how neuroinflammation has been induced and measured in these cultures.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 2