The rhythmic expression of clock genes attenuated in human plaque-derived vascular smooth muscle cells

Lipids in Health and Disease

Research

Ran Zhao¹  Xiaohong Liao¹  Zhu Zhu¹  Ruizhe Qian¹  Changpo Lin²  Junhao Jiang²  Daqiao Guo²  Bin Chen²  Xiao Tang²  Weiguo Fu² 

[1] Department of Physiology and Pathophysiology, Fudan University Shanghai Medical College, 200032, Shanghai, China;Institute of Vascular Surgery, Department of Vascular Surgery, Zhongshan Hospital, Fudan University, 200032, Shanghai, China;

关键词: Circadian rhythm;    Primary cell culture;    Human vascular smooth muscle cells;    Atherosclerosis;    Plaque rupture;   

DOI  :  10.1186/1476-511X-13-14

 received in 2013-11-08, accepted in 2014-01-08,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAcute myocardial infarction and stroke are more likely to occur in the early morning. Circadian pacemakers are considered to be involved in the process. Many peripheral tissues and cells also contain clock systems. In this study, we examined whether the primary cultured human plaque-derived vascular smooth muscle cells (VSMCs) process circadian rhythmicity; furthermore, we investigated the expression difference of clock genes between normal human carotid VSMCs and human plaque-derived VSMCs.MethodsFifty-six human carotid plaques provided the atherosclerotic tissue, and 21 samples yielded viable cultured primary VSMCs. The normal carotid VSMCs were cultured from donors’ normal carotids. The mRNA levels of the target genes were measured by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR).ResultsAfter serum shock, both types of cells showed clear circadian expressions of Bmal1, Cry1, Cry2, Per1, Per2, Per3 and Rev-erbα mRNA; meanwhile the Clock mRNA show a rhythmic expression in plaque-derived SMCs but not in normal carotid VSMCs. The expression levels of these main clock genes were significantly attenuated in human plaque-derived VSMCs compared with normal human carotid VSMCs. The rhythm of Bmal1 mRNA in plaque-derived VSMCs was changed.ConclusionThe present results demonstrate that the human plaque-derived VSMCs possess different circadian rhythmicity from that of normal carotid VSMCs. The rhythm changes of clock genes in plaque-derived VSMCs may be involved in the process of atherosclerosis and finally promote the rupture of plaque.

【 授权许可】

Unknown   
© Lin et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.