Oxidative stress and inflammatory markers in ovarian follicular fluid of women with diminished ovarian reserve during in vitro fertilization

Journal of Ovarian Research

Research

Suyan Xie¹  Dewei Lin¹  Yan Xia¹  Xiaoyan Chen¹  Yan Huang¹  Yexing Xian¹  Xinyu Guo¹  Min Zhang¹  Yi Cheng¹ 

[1] Center of Reproductive Medicine, the General Hospital of Southern Theater Command, 510010, Guangzhou, China;

关键词: Oxidative stress;    Inflammation;    Follicular fluid;    Diminished ovarian reserve;    TNF-α;    IL-18;    GSH;   

DOI  :  10.1186/s13048-023-01293-0

 received in 2023-07-11, accepted in 2023-10-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFollicular microenvironment has been proposed as an important factor for oocyte grown and maturation. We sought to evaluate the oxidative stress and inflammatory levels in follicular fluid (FF) and association with embryo quality in patients with diminished ovarian reserve (DOR).MethodsThe current research included 46 DOR cases and 56 normal ovarian reserve (NOR) cases. Twelve representative oxidative stress markers and eight representative inflammatory factors were measured in the FF.ResultsOxidative stress markers total GSH (T-GSH) was decreased in the FF from women with DOR compared with that in NOR group (P = 0.041). More modest differences were observed for reduced GSH (rGSH) and rGSH/GSSG. Women with DOR compared to controls had higher level of TNF-α (P = 0.000) and lower level of IL-18 (P = 0.013). Correlation analysis revealed that GSSG was negatively correlated with normal fertilization rate in NOR group (r = -0.358, P = 0.008), and reduced GSH was negatively correlated with normal fertilization rate in DOR group (r = -0.299, P = 0.049). Moreover, as the regression analysis data showed, the GSSG level was significantly associated with embryo quality indicator.ConclusionsThe FF in DOR patients was accompanied by increased oxidative stress and inflammatory levels. Follicular development of women with DOR might be influenced by unusual IL-18 and TNF-α levels in FF. And oxidative stress marker GSSG in NOR group was a negative predictor for embryo quality.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023