Intratumor cholesteryl ester accumulation is associated with human breast cancer proliferation and aggressive potential: a molecular and clinicopathological study

BMC Cancer

Research Article

David de Gonzalo-Calvo¹  Lina Badimon¹  Vicenta Llorente-Cortés¹  Laura Nasarre¹  Agusti Barnadas²  Laura López-Vilaró³  Enrique Lerma⁴  Maitane Perez-Olabarria⁵  Daniel Escuin⁵  Tania Vázquez⁵ 

[1] Cardiovascular Research Center, CSIC-ICCC, IIB-Sant Pau, Hospital de la Santa Creu i Sant Pau, Sant Antoni Mª Claret, 167 08025, Barcelona, Spain;Department of Medical Oncology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain;Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallès), Barcelona, Spain;Department of Pathology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain;Institut d’Investigacions Biomèdiques Sant Pau (IIB-Sant Pau), Barcelona, Spain;Department of Pathology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain;Universitat Autònoma de Barcelona (UAB), Bellaterra (Cerdanyola del Vallès), Barcelona, Spain;Institut d’Investigacions Biomèdiques Sant Pau (IIB-Sant Pau), Barcelona, Spain;

关键词: ACAT1;    Breast cancer;    CD36;    Cholesteryl ester;    LDL receptor;    SCARB1;   

DOI  :  10.1186/s12885-015-1469-5

 received in 2014-10-15, accepted in 2015-05-26,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe metabolic effect of intratumor cholesteryl ester (CE) in breast cancer remains poorly understood. The objective was to analyze the relationship between intratumor CE content and clinicopathological variables in human breast carcinomas.MethodsWe classified 30 breast carcinoma samples into three subgroups: 10 luminal-A tumors (ER+/PR+/Her2-), 10 Her-2 tumors (ER-/PR-/Her2+), and 10 triple negative (TN) tumors (ER-/PR-/Her2-). We analyzed intratumor neutral CE, free cholesterol (FC) and triglyceride (TG) content by thin layer chromatography after lipid extraction. RNA and protein levels of lipid metabolism and invasion mediators were analyzed by real time PCR and Western blot analysis.ResultsGroup-wise comparisons, linear regression and logistic regression models showed a close association between CE-rich tumors and higher histologic grade, Ki-67 and tumor necrosis. CE-rich tumors displayed higher mRNA and protein levels of low-density lipoprotein receptor (LDLR) and scavenger receptor class B member 1 (SCARB1). An increased expression of acetyl-Coenzyme A acetyltransferase 1 (ACAT1) in CE-rich tumors was also reported.ConclusionsIntratumor CE accumulation is intimately linked to proliferation and aggressive potential of breast cancer tumors. Our data support the link between intratumor CE content and poor clinical outcome and open the door to new antitumor interventions.

【 授权许可】

Unknown   
© de Gonzalo-Calvo et al. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.