First report on blaNDM-1-producing Acinetobacter baumannii in three clinical isolates from Ethiopia

BMC Infectious Diseases

Research Article

Tefara Belachew¹  Ahmed Zeynudin²  Soeren Schubert³  Maxim Messerer³  Gabriele Liegl³  Thomas Löscher⁴  Andrea Rachow⁵  Michael Pritsch⁵  Michael Hoelscher⁶  Andreas Wieser⁷  Simon Baumer⁸ 

[1] College of Health Sciences, Jimma University, Jimma, Ethiopia;College of Health Sciences, Jimma University, Jimma, Ethiopia;CIH LMU Center for International Health, Ludwig-Maximilians-Universität (LMU), Munich, Germany;Department of Bacteriology, Max von Pettenkofer-Institute (LMU), Munich, Germany;Department of Bacteriology, Max von Pettenkofer-Institute (LMU), Munich, Germany;Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Leopoldstr. 5, 80802, Munich, Germany;Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Leopoldstr. 5, 80802, Munich, Germany;German Center for Infection Research (DZIF), partner site Munich, Munich, Germany;Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Leopoldstr. 5, 80802, Munich, Germany;German Center for Infection Research (DZIF), partner site Munich, Munich, Germany;CIH LMU Center for International Health, Ludwig-Maximilians-Universität (LMU), Munich, Germany;Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU), Leopoldstr. 5, 80802, Munich, Germany;German Center for Infection Research (DZIF), partner site Munich, Munich, Germany;College of Health Sciences, Jimma University, Jimma, Ethiopia;Department of Bacteriology, Max von Pettenkofer-Institute (LMU), Munich, Germany;Faculty of Biology, Ludwig-Maximilians-Universität (LMU), Planegg-Martinsried, Germany;

关键词: NDM-1;    New Delhi metallo-beta-lactamase;    Acinetobacter baumannii;    Carbapenemase;    Ethiopia;    East-Africa;   

DOI  :  10.1186/s12879-017-2289-9

 received in 2016-11-30, accepted in 2017-02-23,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMultidrug-resistant Gram-negative bacterial infections are recognized as one of the major threats to global health. In this study, we describe for the first time blaNDM-1 gene carrying organisms from Ethiopia consisting of three Acinetobacter baumannii isolates from patients in Jimma.MethodsBesides phenotypic antimicrobial susceptibility testing, molecular strain typing and sequencing was performed to describe the phylogenetic relation of the Ethiopian isolates in detail in relation to published isolates from all over the globe.Results and discussionThree multi-resistant, blaNDM-1-positive Acinetobacter baumannii isolates, most likely a local clonal diffusion, were isolated. Two of the three isolates described within this study were untreatable with the locally available antimicrobials and were only susceptible to polymyxin B and amikacin. The genome sequences confirmed the isolates to be distinct from the outbreak strains reported from Kenya, the only other characterized blaNDM-1 positive Acinetobacter baumannii strains in East Africa so far. Up to date, no other bacterial species were found to harbour the gene cassette in Jimma and conjugation to E. coli was not successful under laboratory conditions. However, natural transmission to other bacteria seems likely, given the evident lack of hygienic precautions due to limited resource settings.ConclusionsThe detected isolates could solely be the tip of the iceberg regarding the presence of NDM-1 producing organisms in the region, as only a limited number of bacterial isolates were evaluated so far and until recently, susceptibility testing and isolation of bacteria could hardly be performed in clinical patient care. These multi-drug resistant organisms pose a serious threat to antimicrobial treatments in Jimma, Ethiopia.

【 授权许可】

CC BY   
© The Author(s). 2017