Expression of AEG-1 mRNA and protein in colorectal cancer patients and colon cancer cell lines

Journal of Translational Medicine

Research

Yang-Mei Shen¹  Gunnar Arbman²  Sebastian Gnosa³  Chao-Jie Wang³  Johannes Stratmann³  Xiao-Feng Sun⁴  Hong Zhang⁵ 

[1] Department of Pathology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China;Department of Surgery, Vrinnevi Hospital, Norrköping, Sweden;Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, University of Linköping, Linköping, Sweden;Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, University of Linköping, Linköping, Sweden;Division of Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Country Council of Östergötland, University of Linköping, S-581 85, Linköping, Sweden;Division of Tumour Biology, Systems Biology Research Centre, School of Life Sciences, Skövde University, Skövde, Sweden;

关键词: Liver Metastasis;    Normal Mucosa;    Colon Cancer Cell Line;    Ataxia Telangiectasia Mutate;    Adjacent Normal Mucosa;   

DOI  :  10.1186/1479-5876-10-109

 received in 2012-01-23, accepted in 2012-05-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAstrocyte elevated gene 1 (AEG-1), an important oncogene, has been shown to be overexpressed in several types of cancers. In colorectal cancer (CRC), the protein level of AEG-1 is up-regulated in tumour tissue compared to normal mucosa, showing prognostic significance. Since little is known about the transcriptional level of AEG-1 expression and its biological pathway in CRC the aim of the present study was to examine the relationship of AEG-1 mRNA expression, the protein level and clinicopathological variables as well as its biology pathway in CRC.Material and methodsThe mRNA expression of AEG-1 was analysed by qPCR in fresh frozen patient samples including 156 primary tumours, along with the corresponding normal mucosa, and in five colon cancer cell lines, SW480, SW620, KM12C, KM12SM and KM12L4a. AEG-1 protein expression was investigated by immunohistochemistry in paraffin-embedded materials from 74 distant normal mucosa, 107 adjacent mucosa, 158 primary tumour, 35 lymph node metastasis and 9 liver metastasis samples. In addition, the AEG-1 protein expression was elucidated in the cell lines by Western blot.ResultsThe lymph node metastatic cell line SW620 had a significantly higher AEG-1 mRNA (0.27 ± 0.02) expression compared to the primary tumour cell line SW480 (0.17 ± 0.04, p = 0.026). AEG-1 expression at the mRNA level and/or the protein level was significantly up-regulated gradually from normal mucosa to primary CRC, and then to lymph node metastasis and finally to liver metastasis (p < 0.05). There were significant associations of AEG-1 mRNA expression with tumour location (p = 0.047), as well as mRNA and protein expression with the tumour stage (p < 0.03). Furthermore AEG-1 protein expression was positively related to biological variables including NF-κB, p73, Rad50 and apoptosis (p < 0.05).ConclusionAEG-1 is up-regulated, at the mRNA and the protein level, during CRC development and aggressiveness, and is related to tumour location and stage. It may play its role in CRC through the NF-κB signaling pathway.

【 授权许可】

Unknown   
© Gnosa et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.