Tumor slice culture system to assess drug response of primary breast cancer

BMC Cancer

Research Article

Humberto Sánchez¹  Nicole S. Verkaik¹  Jan H.J. Hoeijmakers¹  Dik C. van Gent¹  Kishan A. T. Naipal¹  Roland Kanaar²  Maaike P.G. Vreeswijk³  Agnes Jager⁴  Carolien H. M. van Deurzen⁵  Michael A. den Bakker⁶ 

[1] Department of Genetics, Cancer Genomics Netherlands, Erasmus University Medical Center, PO box 2040, 3000CA, Rotterdam, The Netherlands;Department of Genetics, Cancer Genomics Netherlands, Erasmus University Medical Center, PO box 2040, 3000CA, Rotterdam, The Netherlands;Department of Radiation Oncology, Erasmus University Medical Center, PO box 2040, 3000CA, Rotterdam, The Netherlands;Department of Human Genetics, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands;Department of Medical Oncology, Erasmus University Medical Center, PO box 2040, 3000CA, Rotterdam, The Netherlands;Department of Pathology, Erasmus University Medical Center, PO box 2040, 3000CA, Rotterdam, The Netherlands;Department of Pathology, Maasstad Hospital, Maasstadweg 21, 3079 DZ, Rotterdam, The Netherlands;

关键词: Breast cancer;    Organotypic tumor tissue slices;    Tissue culture method;    FAC chemotherapy;    Ex vivo sensitivity;   

DOI  :  10.1186/s12885-016-2119-2

 received in 2015-08-10, accepted in 2016-02-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe high incidence of breast cancer has sparked the development of novel targeted and personalized therapies. Personalization of cancer treatment requires reliable prediction of chemotherapy responses in individual patients. Effective selection can prevent unnecessary treatment that would mainly result in the unwanted side effects of the therapy. This selection can be facilitated by characterization of individual tumors using robust and specific functional assays, which requires development of powerful ex vivo culture systems and procedures to analyze the response to treatment.MethodsWe optimized culture methods for primary breast tumor samples that allowed propagation of tissue ex vivo. We combined several tissue culture strategies, including defined tissue slicing technology, growth medium optimization and use of a rotating platform to increase nutrient exchange.ResultsWe could maintain tissue cultures for at least 7 days without losing tissue morphology, viability or cell proliferation. We also developed methods to determine the cytotoxic response of individual tumors to the chemotherapeutic treatment FAC (5-FU, Adriamycin [Doxorubicin] and Cyclophosphamide). Using this tool we designated tumors as sensitive or resistant and distinguished a clinically proven resistant tumor from other tumors.ConclusionThis method defines conditions that allow ex vivo testing of individual tumor responses to anti-cancer drugs and therefore might improve personalization of breast cancer treatment.

【 授权许可】

CC BY   
© Naipal et al. 2016