Globular adiponectin protects H9c2 cells from palmitate-induced apoptosis via Akt and ERK1/2 signaling pathways

Lipids in Health and Disease

Research

An Yu Bao¹  Yan Li¹  Kai Sheng Sun¹  Wei Wu¹  Yong Qing Tong¹  Hong Yun Zheng¹  Wen Dai¹  Chuan Dong Wei² 

[1]Department of Clinical Laboratory, Renmin Hospital of Wuhan University, 99 Ziyang Road, 430060, District of Wuchang, Wuhan, Hubei, China

[2]Department of Clinical Laboratory, Renmin Hospital of Wuhan University, 99 Ziyang Road, 430060, District of Wuchang, Wuhan, Hubei, China

[3]Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for nationalities, 533000, Baise, Guangxi, China

关键词: Adiponectin;    H9c2 cells;    Palmitate;    Apoptosis;    PI3K/Akt;    ERK1/2;   

DOI  :  10.1186/1476-511X-11-135

 received in 2012-07-26, accepted in 2012-09-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCardiomyocytes apoptosis is an important contributor to myocardial dysfunction and heart failure. Adiponectin has cardioprotective effects, potential mechanisms behind it are not clear in cardiomyocytes. The purpose of the study was to investigate whether adiponectin can block palmitate-induced apoptosis and the underlying biochemical mechanism in H9c2 cells.MethodsH9c2 cells were treated with palmitate presence or absence of 2.5 μg/mL globular adiponectin. The effect on the cell viability of H9c2 cells was evaluated using MTT assay, and cell apoptosis was determined by Hoechst 33342 staining. Protein expression was measured using the western blot method.ResultsOur results showed that the palmitate treatment induced apoptosis in H9c2 cells, which was associated with increasing the level of cleaved caspase-3 and cleaved PARP. Meanwhile, palmitate-induced apoptosis increased the protein level of p-ERK1/2, and decreased the protein level of p-Akt significantly. However, levels of both of these proteins were restored to the normal when pretreated with adiponectin, and followed with the decrease of cleaved caspase-3 and cleaved PARP. In line with these results, the protective effect of adiponectin can be blocked by PI3K/Akt inhibitor LY294002, and palmitate-induced apoptosis can be attenuated by ERK1/2 inhibitor U0126.ConclusionsTaken together, the present study demonstrated that adiponectin protects H9c2 cells from palmitate-induced apoptosis via PI3K/Akt and ERK1/2 signaling pathways. Our results reveal a link between adiponectin and cardiomyocytes apoptosis, suggesting that adioponectin may be a promising therapeutic for the treatment of lipotoxicity cardiomyopathy.

【 授权许可】

Unknown   
© Wei et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.