Bioactive lipids are altered in the kidney of chronic undernourished rats: is there any correlation with the progression of prevalent nephropathies?

Lipids in Health and Disease

Reaearch

Valdilene S. Ribeiro¹  Carmem Castro-Chaves¹  Luzia S. Sampaio²  M. Einicker-Lamas³  Paulo A. da Silva⁴  Adalberto Vieyra⁵  Lucienne S. Lara⁶ 

[1] Departamento de Fisiologia e Farmacologia, UFPE, Recife, PE, Brazil;Instituto de Biofísica Carlos Chagas Filho – CCS, UFRJ, Rio de Janeiro, RJ, Brazil;Instituto de Biofísica Carlos Chagas Filho – CCS, UFRJ, Rio de Janeiro, RJ, Brazil;Present Address: Laboratório de Biomembranas, Sala G1-037, Bloco G, Instituto de Biofísica Carlos Chagas Filho – CCS, UFRJ, Ilha do Fundão, 21941-902, Rio de Janeiro, RJ, Brazil;Instituto de Biofísica Carlos Chagas Filho – CCS, UFRJ, Rio de Janeiro, RJ, Brazil;Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Duque de Caxias, RJ, Brazil;Instituto de Biofísica Carlos Chagas Filho – CCS, UFRJ, Rio de Janeiro, RJ, Brazil;Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Duque de Caxias, RJ, Brazil;Centro Nacional de Biologia Estrutural e Bioimagem (CENABIO), UFRJ, Rio de Janeiro, Brazil;Instituto de Ciências Biomédicas, UFRJ, Rio de Janeiro, RJ, Brazil;

关键词: Bioactive lipids;    Cholesterol;    Kidney;    Undernutrition;    Lipid rafts;   

DOI  :  10.1186/s12944-017-0634-z

 received in 2017-08-21, accepted in 2017-12-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundUndernutrition during childhood leads to chronic diseases in adult life including hypertension, diabetes and chronic kidney disease. Here we explore the hypothesis that physiological alterations in the bioactive lipids pattern within kidney tissue might be involved in the progression of chronic kidney disease.MethodsMembrane fractions from kidney homogenates of undernourished rats (RBD) were submitted to lipid extraction and analysis by thin layer chromatography and cholesterol determination.ResultsKidneys from RBD rats had 25% lower cholesterol content, which disturb membrane microdomains, affecting Ca2+ homeostasis and the enzymes responsible for important lipid mediators such as phosphatidylinositol-4 kinase, sphingosine kinase, diacylglicerol kinase and phospholipase A2. We observed a decrease in phosphatidylinositol(4)-phosphate (8.8 ± 0.9 vs. 3.6 ± 0.7 pmol.mg−1.mim−1), and an increase in phosphatidic acid (2.2 ± 0.8 vs. 3.8 ± 1.3 pmol.mg−1.mim−1), being these lipid mediators involved in the regulation of key renal functions. Ceramide levels are augmented in kidney tissue from RBD rats (18.7 ± 1.4 vs. 21.7 ± 1.5 fmol.mg−1.min−1) indicating an ongoing renal lesion.ConclusionResults point to an imbalance in the bioactive lipid generation with further consequences to key events related to kidney function, thus contributing to the establishment of chronic kidney disease.

【 授权许可】

CC BY   
© The Author(s). 2017