In vitro culture of Plasmodium berghei-ANKA maintains infectivity of mouse erythrocytes inducing cerebral malaria

Malaria Journal

Methodology

Valery Combes¹  Georges E Grau¹  Fatima El-Assaad¹  Ronan Jambou² 

[1] Vascular Immunology Unit, Department of Pathology and Bosch Institute, Sydney Medical School, The University of Sydney, NSW, Australia;Vascular Immunology Unit, Department of Pathology and Bosch Institute, Sydney Medical School, The University of Sydney, NSW, Australia;Institut Pasteur de Madagascar, BP1274, Antananarivo 101, Antananarivo, Madagascar;

关键词: Malaria;    Cerebral Malaria;    Plasmodium Berghei;    Murine Malaria;    Gametocyte Production;   

DOI  :  10.1186/1475-2875-10-346

 received in 2011-10-29, accepted in 2011-11-25,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundInfection with Plasmodium berghei is a widely used model of murine malaria and a powerful tool for reverse genetic and pathogenesis studies. However, the efficacy of in vitro reinvasion of erythrocytes is generally low, limiting in vitro studies.MethodsPlasmodium berghei ANKA-infected blood obtained from a susceptible infected mouse was cultured in various conditions and in vitro parasitaemia was measured every day to evaluate the rate of reinvasion.ResultsHigh quality culture media were used and reinvasion rates were improved by vigorous orbital shaking of the flask and increasing density of the medium with gelatin.DiscussionUsing these settings, reinvasion of normal mouse erythrocytes by the parasite was obtained in vitro over two weeks with preservation of the infectivity in vivo.

【 授权许可】

CC BY   
© Jambou et al; licensee BioMed Central Ltd. 2011