Screening for atypical porcine pestivirus in Swedish boar semen used for artificial insemination and a characterisation of the seminal RNA microbiome including the virome

BMC Veterinary Research

Research

Hedvig Stenberg¹  Maja Malmberg¹  Juliette Hayer² 

[1] Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, SLU, P.O. Box 7028, 750 07, Uppsala, Sweden;MIVEGEC, University of Montpellier, IRD, CNRS, Montpellier, France;

关键词: Atypical porcine pestivirus;    Congenital tremor;    Semen;    Breeding boars;    Artificial insemination;    Seminal;    Virome;    Microbiome;    Pigs;    High-throughput sequencing;   

DOI  :  10.1186/s12917-023-03762-6

 received in 2023-05-19, accepted in 2023-09-30,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThis study aimed to characterise the RNA microbiome, including the virome of extended semen from Swedish breeding boars, with particular focus on Atypical porcine pestivirus (APPV). This neurotropic virus, associated with congenital tremor type A-II in piglets, was recently demonstrated to induce the disease through insemination with semen from infected boars.ResultsFrom 124 Artificial Insemination (AI) doses from Swedish breeding boars, APPV was detected in one dose in addition to a sparse seminal RNA virome, characterised by retroviruses, phages, and some fecal-associated contaminants. The detected seminal microbiome was large and characterized by Gram-negative bacteria from the phylum Proteobacteria, mainly consisting of apathogenic or opportunistic bacteria. The proportion of bacteria with a pathogenic potential was low, and no antimicrobial resistance genes (ARGs) were detected in the datasets.ConclusionOverall, the results indicate a good health status among Swedish breeding boars. The detection of APPV in semen raises the question of whether routine screening for APPV in breeding boars should be instigated.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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