Astrovirus as a possible cause of congenital tremor type AII in piglets?

Acta Veterinaria Scandinavica

Anne-Lie Blomström¹  Cecilia Ley¹  Magdalena Jacobson² 

[1] Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, SE-750 07, Sweden

[2] Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, SE-750 07, Sweden

关键词: Myelin vacuolation;    Congenital tremor;    Circovirus;    Astrovirus;   

Others  :  1082513
DOI  :  10.1186/s13028-014-0082-y

 received in 2014-05-14, accepted in 2014-11-24,  发布年份 2014

【 摘 要 】

Background

Congenital tremor is associated with demyelination of the brain and spinal cord and is clinically noted as outbreaks of trembling and shaking in newborn piglets during a limited time-period. Six forms of the disease have been described, where form AII may be caused by an, as yet, unidentified viral infection. This study aimed to investigate the presence of astrovirus and circovirus by sequencing and polymerase chain reaction (PCR) analysis and by relating the findings to the occurrence of disease and lesions in the brain, in 4–6 days-old piglets obtained from a clinical outbreak of congenital tremor.

Results

In piglets with congenital tremor, there were mild to moderate vacuolar changes of the white matter in the cerebrum, brain stem and cerebellum. In healthy piglets, less conspicuous vacuolar changes were detected. One healthy and one diseased piglet were positive for porcine circovirus type 2. The nested pan-PCR showed the presence of astrovirus in at least one brain region in all piglets and by sequencing, two different porcine astrovirus lineages were identified.

Conclusions

The results do not support previous studies identifying porcine circovirus type 2 as the cause of congenital tremor. The demonstration of astrovirus in the brain of piglets suffering from congenital tremor is interesting. However, astrovirus was demonstrated in both healthy and diseased individuals and therefore, further studies are warranted to determine the possible involvement of astrovirus in the pathogenesis of congenital tremor in pigs.

【 授权许可】

   
2014 Blomström et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Kinsley AT: Dancing pigs? Vet Med 1922, 17:123.
• [2]Bölske G, Kronevi T, Lindgren NO: Congential tremor in pigs in Sweden. Nord Vet Med J 1978, 30:534-537.
• [3]Done JT: Congenital nervous diseases of pigs: A review. Lab Animal 1968, 2:207-217.
• [4]Done JT: The congenital tremor syndrome in pigs. Vet Annual 1976, 16:98-102.
• [5]Done S, Williamson SM, Strugnell BW: Nervous and locomotor systems. In Diseases of Swine. 10th edition. Edited by Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW. Wiley – Blackwell, Iowa, USA; 2012:304-305.
• [6]Larsson EL: Om skaksjuka hos smågrisar. Sv Svinavelsför Tidskrft 1955, 9:149-151.
• [7]Done JT, Woolley J, Upcott DH, Hebert CN: Porcine congenital tremor type AII: spinal cord morphometry. Br Vet J 1986, 142:145-150.
• [8]Hines RK and Lukert PD: Porcine circovirus as a cause of congenital tremors in newborn pigs. In: Proceedings of the Annual Meeting of the American Association of Swine Practitioners: Chicago, USA, 1994:344–345.
• [9]Stevenson GW, Kiupel M, Mittal SK, Choi J, Latimer KS, Kanitz CL: Tissue distribution and genetic typing of porcine circovirus in pigs with naturally occurring congenital tremors. J Vet Diagn Invest 2001, 13:57-62. doi:10.1177/104063870101300111
• [10]Kennedy S, Segalés J, Rovira A, Scholes S, Domingo M, Moffett D, Meehan B, O’Neill R, McNeilly F, Allan G: Absence of evidence of porcine circovirus infection in piglets with congenital tremors. J Vet Diagn Invest 2003, 15:151-156. doi:10.1177/104063870301500209
• [11]Chae C: A review of porcine circovirus 2-associated syndromes and diseases. Vet J 2005, 169:326-336.
• [12]Christensen E, Christensen NO: Studies on “trembling in new-born pigs”. Nord Vet-Med 1956, 8:921-934.
• [13]Blomström A-L, Widén F, Hammer A-S, Belák S, Berg M: Detection of a novel astrovirus in brain tissue of mink suffering from shaiking mink syndrome using viral metagenomics. J Clin Microbiol 2010, 48:4392-4396. doi:10.1128/JCM.01040-10
• [14]Shimizu M, Shirai J, Narita M, Yamane T: Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney. J Clin Microbiol 1990, 28:201-206.
• [15]Brnić D, Prpić J, Keros T, Roić B, Starešina V, Jemeršić L: Porcine astrovirus viremia and high genetic variability in pigs on large scale holdings in Croatia. Infect Genet Evol 2013, 14:258-264.
• [16]Blomström A-L, Belák S, Fossum C, Fuxler L, Wallgren P, Berg M: Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. Vir Res 2010, 152:59-64. doi:10.1016/j.virusres.2010.06.004
• [17]Chu DK, Poon LL, Guan Y, Peiris JS: Novel astroviruses in insectivorous bats. J Virol 2008, 82:9107-9114. doi:10.1128/JVI.00857-08
• [18]Kapoor A, Li L, Victoria J, Oderinde B, Mason C, Pandey P, Zaidi SZ, Delwart E: Multiple novel astrovirus species in human stool. J Gen Virol 2009, 90:2965-2972. doi:10.1099/vir.0.014449-0
• [19]Laurin MA, Dastor M, L’homme Y: Detection and genetic characterization of a novel pig astrovirus: relationship to other astroviruses. Arch Virol 2011, 156:2095-2099. doi:10.1007/s00705-011-1088-7
• [20]Luo Z, Roi S, Dastor M, Gallice E, Laurin MA, L’homme Y: Multiple novel and prevalent astroviruses in pigs. Vet Microbiol 2011, 149:316-323. doi:10.1016/j.vetmic.2010.11.026
• [21]Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596-1599.
• [22]de Benedictis P, Schultz-Cherry S, Burnham A, Cattoli G: Astrovirus infections in humans and animals - molecular biology, genetic diversity, and interspecies transmissions. Infect Genet Evol 2011, 11:1529-1544. doi:10.1016/j.meegid.2011.07.024]
• [23]Bridger JC: Detection by electron microscopy of caliciviruses, astroviruses and rota-virus-like particles in the faeces of piglets with diarrhoea. Vet Rec 1980, 107:532-533.
• [24]Quan PL, Wagner TA, Briese T, Torgerson TR, Hornig M, Tashmukhamedova A, Firth C, Palacios G, Baisre-De-Leon A, Paddock CD, Hutchison SK, Egholm M, Zaki SR, Goldman J, Ochs HD, Lipkin WI: Astrovirus encephalitis in boy with X-linked agammaglobulinemia. Emerg Inf Dis 2010, 16:918-925. doi:10.3201/eid1606.091536
• [25]Maxie MG, Youssef S: Nervous System. In Jubb, Kennedy and Palmer’s Pathology of Domestic Animals, Volume 1. 5th edition. Edited by Maxie MG. Saunders Elsevier, Philadelphia, PA, USA; 2007:385-386.
• [26]McInnes E: Artefacts in histology. Comp Clin Pathol 2005, 13:100-108.
• [27]Balantine JD, Greene WB: Vesiculation of myelin in postmortem autolysis. J Neuropathol Exp Neurol 1986, 45:140.
• [28]Torello LA, Yates AJ, Horrocks LA: Effects of postmortem autolysis on the lipids of rat spinal cord. Virchows Arch B: Cell Pathol 1981, 13:219-238.
• [29]Yates AJ, Thelmo W, Pappius H: Postmortem changes in the chemistry and histology of normal and edematous brains. Am J Pathol 1975, 79:555-564.