Identification of a novel SEREX antigen family, ECSA, in esophageal squamous cell carcinoma

Proteome Science

Research

Mari Yasuraoka¹  Masaki Takiguchi¹  Takaki Hiwasa¹  Mari Kuboshima²  Akiko Kagaya²  Kazue Nakashima-Fujita²  Yutaka Tamura³  Hisahiro Matsubara⁴  Tooru Shiratori⁴  Takanori Nishimori⁴  Fumio Nomura⁵  Hideaki Shimada⁶  Shunsuke Kurei⁶  Takenori Ochiai⁷  Akihiro Murakami⁸  Takahisa Hachiya⁸ 

[1] Department of Biochemistry and Genetics, Chiba University, Graduate School of Medicine, 260-8670, Chuo-ku, Chiba, Japan;Department of Biochemistry and Genetics, Chiba University, Graduate School of Medicine, 260-8670, Chuo-ku, Chiba, Japan;Department of Frontier Surgery, Chiba University, Graduate School of Medicine, 260-8670, Chuo-ku, Chiba, Japan;Department of Bioinfomatics, Chiba University, Graduate School of Medicine, 260-8670, Chuo-ku, Chiba, Japan;Department of Frontier Surgery, Chiba University, Graduate School of Medicine, 260-8670, Chuo-ku, Chiba, Japan;Department of Molecular Diagnosis, Chiba University, Graduate School of Medicine, 260-8670, Chuo-ku, Chiba, Japan;Department of Surgery, School of Medicine, Toho University, 143-8541, Ota-ku, Tokyo, Japan;Gastroenterological Center, San-Ai Memorial Hospital, 260-0806, Chuo-ku, Chiba, Japan;Product Development Department, Medical & Biological Laboratories Co., Ltd., Ina, 396-0002, Nagano, Japan;

关键词: Esophageal Squamous Cell Carcinoma;    Healthy Donor;    Esophageal Carcinoma;    Esophageal Squamous Cell Carcinoma Tissue;    Esophageal Squamous Cell Carcinoma Cell Line;   

DOI  :  10.1186/1477-5956-9-31

 received in 2010-11-26, accepted in 2011-06-23,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundDiagnosis of esophageal squamous cell carcinoma (SCC) may improve with early diagnosis. Currently it is difficult to diagnose SCC in the early stage because there is a limited number of tumor markers available.ResultsFifty-two esophageal SCC SEREX antigens were identified by SEREX (serological identification of antigens by recombinant cDNA expression cloning) using a cDNA phage library and sera of patients with esophageal SCC. Sequence analysis revealed that three of these antigens were similar in amino acid sequences, and they were designated as ECSA (e sophageal c arcinoma S EREX a ntigen)-1, -2 and -3. The ECSA family was also similar to an EST clone, hepatocellular carcinoma-associated antigen 25a (HCA25a). Serum antibody levels to ECSA-1, -2 and -3 were significantly higher in patients with esophageal SCC than in healthy donors. Based on the conserved amino acid sequences, three peptides were synthesized and used for enzyme-linked immunosorbent assays (ELISA). The serum antibody levels against one of these peptides were significantly higher in patients with esophageal SCC. This peptide sequence was also conserved in FAM119A, GOSR1 and BBS5, suggesting that these are also ECSA family members. Reverse transcription followed by quantitative PCR analysis showed that the mRNA expression levels of ECSA-1, -2 and -3 and FAM119A but not of HCA25a, GOSR1 and BBS5 were frequently elevated in esophageal SCC tissues.ConclusionsWe have identified a new gene family designated ECSA. Serum antibodies against the conserved domain of the ECSA family may be a promising tumor marker for esophageal SCC.

【 授权许可】

Unknown   
© Kagaya et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.