An innovative tool for moving malaria PCR detection of parasite reservoir into the field

Malaria Journal

Research

Saorin Kim¹  Kaknika Loch¹  Nimol Khim¹  Lydie Canier¹  Sophy Chy¹  Malen Ken¹  Chanra Khean¹  Pascal Masse-Navette¹  Hokkean Lim¹  Dany Dourng¹  Rotha Eam¹  Didier Ménard¹  Vincent Deubel¹  Somony Heng²  Lies Durnez³  Charlotte Gryseels³  Koen Peeters Grietens³  Vincent Sluydts³  Karel Van Roey³  Marc Coosemans⁴  Sambunny Uk⁵  Sovannaroath Siv⁵  Sochantha Tho⁵  Boukheng Thavrin⁵  Mao Sokny⁵  Char Meng Chuor⁵ 

[1] Institut Pasteur du Cambodge, Phnom Penh, Cambodia;Institut Pasteur du Cambodge, Phnom Penh, Cambodia;Institute of Tropical Medicine, Antwerp, Belgium;Institute of Tropical Medicine, Antwerp, Belgium;Institute of Tropical Medicine, Antwerp, Belgium;Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium;National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia;

关键词: Malaria;    Rapid Diagnostic Test;    Mobile Laboratory;    Malaria Elimination;    Malaria Species;   

DOI  :  10.1186/1475-2875-12-405

 received in 2013-10-02, accepted in 2013-11-05,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundTo achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field.MethodsDNA extraction and real-time PCR assays were implemented in an “in-house” designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831).ResultsThe real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Rattanakiri Province, to screen approximately 5,000 individuals in less than four weeks and treat parasite carriers within 24–48 hours after sample collection. An average of 240 clinical samples (and 40 quality control samples) was tested every day, six/seven days per week. Some 97.7% of the results were available <24 hours after the collection. A total of 4.9% were positive for malaria. Plasmodium vivax was present in 61.1% of the positive samples, Plasmodium falciparum in 45.9%, Plasmodium malariae in 7.0% and Plasmodium ovale in 2.0%.ConclusionsThe operational success of this diagnostic set-up proved that molecular testing and subsequent treatment is logistically achievable in field settings. This will allow the detection of clusters of asymptomatic carriers and to provide useful epidemiological information. Fast results will be of great help for staff in the field to track and treat asymptomatic parasitaemic cases. The concept of the mobile laboratory could be extended to other countries for the molecular detection of malaria or other pathogens, or to culture vivax parasites, which does not support long-time delay between sample collection and culture.

【 授权许可】

Unknown   
© Canier et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.