Genome-wide characterization of vibrio phage ϕpp2 with unique arrangements of the mob-like genes

BMC Genomics

Research Article

Ying-Rong Lin¹  Chan-Shing Lin² 

[1] Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 80424, Kaohsiung, Taiwan;Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, 80424, Kaohsiung, Taiwan;Agricultural Biotechnology Center, National Chung-Hsing University, 402, Taichung, Taiwan;

关键词: Homing endonuclease;    T4-like phage;    Vibrio parahaemolyticus;   

DOI  :  10.1186/1471-2164-13-224

 received in 2011-10-10, accepted in 2012-05-07,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundVibrio parahaemolyticus is associated with gastroenteritis, wound infections, and septicemia in human and animals. Phages can control the population of the pathogen. So far, the only one reported genome among giant vibriophages is KVP40: 244,835 bp with 26% coding regions that have T4 homologs. Putative homing endonucleases (HE) were found in Vibrio phage KVP40 bearing one seg D and Vibrio cholerae phage ICP1 carrying one mob C/E and one seg G.ResultsA newly isolated Vibrio phage ϕpp2, which was specific to the hosts of V. parahaemolyticus and V. alginolyticus, featured a long nonenveloped head of ~90 × 150 nm and tail of ~110 nm. The phage can survive at 50°C for more than one hour. The genome of the phage ϕpp2 was sequenced to be 246,421 bp, which is 1587 bp larger than KVP40. 383 protein-encoding genes (PEGs) and 30 tRNAs were found in the phage ϕpp2. Between the genomes of ϕpp2 and KVP40, 254 genes including 29 PEGs for viral structure were of high similarity, whereas 17 PEGs of KVP40 and 21 PEGs of ϕpp2 were unmatched. In both genomes, the capsid and tail genes have been identified, as well as the extensive representation of the DNA replication, recombination, and repair enzymes. In addition to the three giant indels of 1098, 1143 and 3330 nt, ϕpp2 possessed unique proteins involved in potassium channel, gp2 (DNA end protector), tRNA nucleotidyltransferase, and mob-type HEs, which were not reported in KVP40. The ϕpp2 PEG274, with strong promoters and translational initiation, was identified to be a mob E type, flanked by NrdA and NrdB/C homologs. Coincidently, several pairs of HE-flanking homologs with empty center were found in the phages of Vibrio phages ϕpp2 and KVP40, as well as in Aeromonas phages (Aeh1 and Ae65), and cyanophage P-SSM2.ConclusionsVibrio phage ϕpp2 was characterized by morphology, growth, and genomics with three giant indels and different types of HEs. The gene analysis on the required elements for transcription and translation suggested that the ϕpp2 PEG274 was an active mob E gene. The phage was signified to be a new species of T4-related, differing from KVP40.

【 授权许可】

CC BY   
© Lin and Lin; licensee BioMed Central Ltd. 2012