Rapid compensatory changes in the expression of EAAT-3 and GAT-1 transporters during seizures in cells of the CA1 and dentate gyrus

Journal of Biomedical Science

Research

Laura Medina-Ceja¹  Silvia J López-Pérez¹  Flavio Sandoval-García¹  Alberto Morales-Villagrán¹ 

[1] Laboratorio de Neurofisiología y Neuroquímica, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Km. 15.5 Carretera Guadalajara-Nogales Predio “Las Agujas”m Nextipac, CP 45110, Zapopan, Jalisco, Mexico;

关键词: 4-Aminopyridine;    EAAT-3;    GAT-1;    Hippocampus;    Immunofluorescence;    Seizures;   

DOI  :  10.1186/1423-0127-19-78

 received in 2012-04-04, accepted in 2012-08-21,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEpilepsy is a neurological disorder produced by an imbalance between excitatory and inhibitory neurotransmission, in which transporters of both glutamate and GABA have been implicated. Hence, at different times after local administration of the convulsive drug 4-aminopyridine (4-AP) we analyzed the expression of EAAT-3 and GAT-1 transporter proteins in cells of the CA1 and dentate gyrus.MethodsDual immunofluorescence was used to detect the co-localization of transporters and a neuronal marker. In parallel, EEG recordings were performed and convulsive behavior was rated using a modified Racine Scale.ResultsBy 60 min after 4-AP injection, EAAT-3/NeuN co-labelling had increased in dentate granule cells and decreased in CA1 pyramidal cells. In the latter, this decrease persisted for up to 180 min after 4-AP administration. In both the DG and CA1, the number of GAT-1 labeled cells increased 60 min after 4-AP administration, although by 180 min GAT-1 labeled cells decreased in the DG alone. The increase in EAAT-3/NeuN colabelling in DG was correlated with maximum epileptiform activity and convulsive behavior.ConclusionsThese findings suggest that a compensatory mechanism exists to protect against acute seizures induced by 4-AP, whereby EAAT-3/NeuN cells is rapidly up regulated in order to enhance the removal of glutamate from the extrasynaptic space, and attenuating seizure activity.

【 授权许可】

CC BY   
© Medina-Ceja et al.; licensee BioMed Central Ltd. 2012