MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells

BMC Genomics

Research Article

Pierre-Benoit Ancey¹  Zdenko Herceg¹  Maria Ouzounova¹  Mylène Ferrand¹  Hector Hernandez-Vargas²  Chantal Matar³  Tri Vuong³  Florence Le-Calvez Kelm⁴  Geoffroy Durand⁴  Carlo Croce⁵ 

[1] Epigenetics Group. International Agency for Research on Cancer (IARC), 150 rue Albert-Thomas, 69008, Lyon, France;Epigenetics Group. International Agency for Research on Cancer (IARC), 150 rue Albert-Thomas, 69008, Lyon, France;Epigenetics Group, International Agency for Research on Cancer (IARC), 150 cours Albert-Thomas, 69372, Lyon cedex 08, France;Faculty of Health Sciences, University of Ottawa, K1H 8M5, Ottawa, Canada;Genetic Cancer Susceptibility Group, International Agency for Research on Cancer (IARC), 150 rue Albert-Thomas, 69008, Lyon, France;Ohio State University, 1082 Biomedical Research Tower, 460 W 12th Ave, 43210, Columbus, OH, USA;

关键词: Breast cancer;    BT-ICs;    Mammospheres;    microRNAs;    miR-30 family;    AVEN;   

DOI  :  10.1186/1471-2164-14-139

 received in 2012-09-08, accepted in 2013-02-23,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundA subset of breast cancer cells displays increased ability to self-renew and reproduce breast cancer heterogeneity. The characterization of these so-called putative breast tumor-initiating cells (BT-ICs) may open the road for novel therapeutic strategies. As microRNAs (miRNAs) control developmental programs in stem cells, BT-ICs may also rely on specific miRNA profiles for their sustained activity. To explore the notion that miRNAs may have a role in sustaining BT-ICs, we performed a comprehensive profiling of miRNA expression in a model of putative BT-ICs enriched by non-attachment growth conditions.ResultsWe found breast cancer cells grown under non-attachment conditions display a unique pattern of miRNA expression, highlighted by a marked low expression of miR-30 family members relative to parental cells. We further show that miR-30a regulates non-attachment growth. A target screening revealed that miR-30 family redundantly modulates the expression of apoptosis and proliferation-related genes. At least one of these targets, the anti-apoptotic protein AVEN, was able to partially revert the effect of miR-30a overexpression. Finally, overexpression of miR-30a in vivo was associated with reduced breast tumor progression.ConclusionsmiR30-family regulates the growth of breast cancer cells in non-attachment conditions. This is the first analysis of target prediction in a whole family of microRNAs potentially involved in survival of putative BT-ICs.

【 授权许可】

Unknown   
© Ouzounova et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.