The core genome of the anaerobic oral pathogenic bacterium Porphyromonas gingivalis

BMC Microbiology

Research Article

Wim Crielaard¹  Marja L Laine¹  Johannes J de Soet¹  Jorg Brunner¹  Timo M Breit²  Floyd RA Wittink²  Martijs J Jonker²  Mark de Jong² 

[1] Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Free University Amsterdam, Amsterdam, The Netherlands;Microarray Department and Integrative Bioinformatics Unit, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands;

关键词: Periodontitis;    Test Strain;    Core Genome;    Cluster Regularly Interspaced Short Palindromic Repeat;    Aberrant Gene;   

DOI  :  10.1186/1471-2180-10-252

 received in 2010-06-10, accepted in 2010-09-29,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe Gram negative anaerobic bacterium Porphyromonas gingivalis has long been recognized as a causative agent of periodontitis. Periodontitis is a chronic infectious disease of the tooth supporting tissues eventually leading to tooth-loss. Capsular polysaccharide (CPS) of P. gingivalis has been shown to be an important virulence determinant. Seven capsular serotypes have been described. Here, we used micro-array based comparative genomic hybridization analysis (CGH) to analyze a representative of each of the capsular serotypes and a non-encapsulated strain against the highly virulent and sequenced W83 strain. We defined absent calls using Arabidopsis thaliana negative control probes, with the aim to distinguish between aberrations due to mutations and gene gain/loss.ResultsOur analyses allowed us to call aberrant genes, absent genes and divergent regions in each of the test strains. A conserved core P. gingivalis genome was described, which consists of 80% of the analyzed genes from the sequenced W83 strain. The percentage of aberrant genes between the test strains and control strain W83 was 8.2% to 13.7%. Among the aberrant genes many CPS biosynthesis genes were found. Most other virulence related genes could be found in the conserved core genome. Comparing highly virulent strains with less virulent strains indicates that hmuS, a putative CobN/Mg chelatase involved in heme uptake, may be a more relevant virulence determinant than previously expected. Furthermore, the description of the 39 W83-specific genes could give more insight in why this strain is more virulent than others.ConclusionAnalyses of the genetic content of the P. gingivalis capsular serotypes allowed the description of a P. gingivalis core genome. The high resolution data from three types of analysis of triplicate hybridization experiments may explain the higher divergence between P. gingivalis strains than previously recognized.

【 授权许可】

Unknown   
© Brunner et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.