Comparative analysis and supragenome modeling of twelve Moraxella catarrhalis clinical isolates

BMC Genomics

Research Article

Josh Earl¹  Azad Ahmed¹  Garth D Ehrlich²  Fen Z Hu²  John P Hays³  Jeremiah J Davie⁴  Anthony A Campagnari⁵  Robert M Wadowsky⁶  Hester J Bootsma⁷  Peter WM Hermans⁷  Kim Stol⁷  Stefan PW de Vries⁷ 

[1] Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, USA;Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, USA;Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, USA;Department of Otolaryngology-Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, USA;Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre (Erasmus MC), Rotterdam, The Netherlands;Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York, USA;Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York, USA;Department of Medicine, Division of Infectious Disease, University at Buffalo, Buffalo, New York, USA;Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, Buffalo, New York, USA;Department of Pathology, School of Medicine, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA;Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA;Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA;Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands;

关键词: Chronic Obstructive Pulmonary Disease;    Otitis Medium;    Codon Usage;    Core Genome;    Cluster Regularly Interspaced Short Palindromic Repeat;   

DOI  :  10.1186/1471-2164-12-70

 received in 2010-11-04, accepted in 2011-01-26,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundM. catarrhalis is a gram-negative, gamma-proteobacterium and an opportunistic human pathogen associated with otitis media (OM) and exacerbations of chronic obstructive pulmonary disease (COPD). With direct and indirect costs for treating these conditions annually exceeding $33 billion in the United States alone, and nearly ubiquitous resistance to beta-lactam antibiotics among M. catarrhalis clinical isolates, a greater understanding of this pathogen's genome and its variability among isolates is needed.ResultsThe genomic sequences of ten geographically and phenotypically diverse clinical isolates of M. catarrhalis were determined and analyzed together with two publicly available genomes. These twelve genomes were subjected to detailed comparative and predictive analyses aimed at characterizing the supragenome and understanding the metabolic and pathogenic potential of this species. A total of 2383 gene clusters were identified, of which 1755 are core with the remaining 628 clusters unevenly distributed among the twelve isolates. These findings are consistent with the distributed genome hypothesis (DGH), which posits that the species genome possesses a far greater number of genes than any single isolate. Multiple and pair-wise whole genome alignments highlight limited chromosomal re-arrangement.ConclusionsM. catarrhalis gene content and chromosomal organization data, although supportive of the DGH, show modest overall genic diversity. These findings are in stark contrast with the reported heterogeneity of the species as a whole, as wells as to other bacterial pathogens mediating OM and COPD, providing important insight into M. catarrhalis pathogenesis that will aid in the development of novel therapeutic regimens.

【 授权许可】

Unknown   
© Davie et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.