Antibacterial activity of extracellular compounds produced by a Pseudomonas strain against methicillin-resistant Staphylococcus aureus (MRSA) strains

Annals of Clinical Microbiology and Antimicrobials

Research

Célia GTJ Andrade¹  Wanderley D Silveira²  Renata KT Kobayashi³  Galdino Andrade³  Admilton G Oliveira³  Viviane F Cardozo³  Gerson Nakazato³  Erick K Nishio³  Marcia RE Perugini⁴  Nelson Durán⁵ 

[1] Department of General Biology, University of Londrina State, CP 86051-990, Londrina, PR, Brazil;Department of Genetics, Evolution and Bioagent, Biology Institute, Campinas State University (UNICAMP), CP 13083-970, Campinas, SP, Brazil;Department of Microbiology, Biology Sciences Center, University of Londrina State, CP 86005-990, Londrina, PR, Brazil;Department of Pathology, Clinical Analysis and Toxicological, University of Londrina State, CP 86038-440, Londrina, PR, Brazil;Department of Physical Chemistry, Chemistry Institute Campinas State University (UNICAMP), CP 13083-970, Campinas, SP, Brazil;

关键词: Antibacterial activity;    Methicillin-resistant;    Pseudomonas aeruginosa;    Staphylococcus aureus;   

DOI  :  10.1186/1476-0711-12-12

 received in 2013-03-01, accepted in 2013-06-08,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe emergence of multidrug-resistant bacteria is a world health problem. Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA) strains, is one of the most important human pathogens associated with hospital and community-acquired infections. The aim of this work was to evaluate the antibacterial activity of a Pseudomonas aeruginosa-derived compound against MRSA strains.MethodsThirty clinical MRSA strains were isolated, and three standard MRSA strains were evaluated. The extracellular compounds were purified by vacuum liquid chromatography. Evaluation of antibacterial activity was performed by agar diffusion technique, determination of the minimal inhibitory concentration, curve of growth and viability and scanning electron microscopy. Interaction of an extracellular compound with silver nanoparticle was studied to evaluate antibacterial effect.ResultsThe F3 (ethyl acetate) and F3d (dichloromethane- ethyl acetate) fractions demonstrated antibacterial activity against the MRSA strains. Phenazine-1-carboxamide was identified and purified from the F3d fraction and demonstrated slight antibacterial activity against MRSA, and synergic effect when combined with silver nanoparticles produced by Fusarium oxysporum. Organohalogen compound was purified from this fraction showing high antibacterial effect. Using scanning electron microscopy, we show that the F3d fraction caused morphological changes to the cell wall of the MRSA strains.ConclusionsThese results suggest that P. aeruginosa-produced compounds such as phenazines have inhibitory effects against MRSA and may be a good alternative treatment to control infections caused by MRSA.

【 授权许可】

Unknown   
© Cardozo et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.