Assembly and analysis of a male sterile rubber tree mitochondrial genome reveals DNA rearrangement events and a novel transcript

BMC Plant Biology

Research Article

Thippawan Yoocha¹  Somvong Tragoonrung¹  Sithichoke Tangphatsornruang¹  Duangjai Sangsrakru¹  Pichahpuk Uthaipaisanwong¹  Panthita Ruang-areerate¹  Supannee Poopear¹  Chutima Sonthirod¹  Jeremy R Shearman¹  Kanikar Theerawattanasuk² 

[1] National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Road, Khlong Nueng, 12120, Khlong Luang, Pathumthani, Thailand;Rubber Research Institute of Thailand (RRIT), Department of Agriculture, Ministry of Agriculture and Cooperatives, 50 Phaholyothin Road, 10900, Chatuchack, Bangkok, Thailand;

关键词: Rubber tree;    Hevea brasiliensis;    Mitochondria;    Cytoplasmic male sterility;    Genome sequencing;   

DOI  :  10.1186/1471-2229-14-45

 received in 2013-12-20, accepted in 2014-02-07,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe rubber tree, Hevea brasiliensis, is an important plant species that is commercially grown to produce latex rubber in many countries. The rubber tree variety BPM 24 exhibits cytoplasmic male sterility, inherited from the variety GT 1.ResultsWe constructed the rubber tree mitochondrial genome of a cytoplasmic male sterile variety, BPM 24, using 454 sequencing, including 8 kb paired-end libraries, plus Illumina paired-end sequencing. We annotated this mitochondrial genome with the aid of Illumina RNA-seq data and performed comparative analysis. We then compared the sequence of BPM 24 to the contigs of the published rubber tree, variety RRIM 600, and identified a rearrangement that is unique to BPM 24 resulting in a novel transcript containing a portion of atp9.ConclusionsThe novel transcript is consistent with changes that cause cytoplasmic male sterility through a slight reduction to ATP production efficiency. The exhaustive nature of the search rules out alternative causes and supports previous findings of novel transcripts causing cytoplasmic male sterility.

【 授权许可】

Unknown   
© Shearman et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.