Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema

Journal of Inflammation

Research

Jianmei Cai¹  Xuejun Sun¹  Chunlei Jiang¹  Zhen Zhu¹  Zheng Xu¹  Jiangrui Zhou¹ 

[1] Department of Nautical Medicine, Second Military Medical University, 800 Xiangyin Road, 200433, Shanghai, P.R. of China;

关键词: hydrogen saline;    macrophages;    TNF-α;    anti-inflammation;    carrageenan;   

DOI  :  10.1186/1476-9255-9-2

 received in 2010-08-20, accepted in 2012-02-02,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundOxidative stress is thought to play an important role in the pathogenesis of inflammation. Recent studies have found that hydrogen gas has the effect of eliminating free radicals. Whether hydrogen saline (more convenient to be used than hydrogen gas) has the anti-inflammation effect or not is still unknown.MethodsCarrageenan-induced paw oedema and LPS-activated macrophages are studied in this article. Injection of carrageenan into the foot of a mouse elicited an acute inflammatory response characterized by increase of foot volume and infiltration of neutrophils. While tumor necrosis factorα(TNF-α) secreted by activated macrophages was determined by ELISA and real-time PCR.ResultsAll parameters of inflammation (foot volume, infiltration of neutrophils, amount of TNF-α and the level of TNF-α's mRNA) were attenuated by the hydrogen saline treatment.ConclusionAs a more convenient way than inhaling H2, hydrogen saline exhibits a protective effect against inflammation and it might provide a novel therapeutic approach for inflammatory diseases.

【 授权许可】

Unknown   
© Xu et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.