Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites

Journal of Nanobiotechnology

Research

Yasushi Miyahira¹  Takeshi Ono¹  Jun Kinoda²  Takemi Matsui³  Vinh Quang Nguyen⁴  Hidemi Hattori⁵  Shingo Nakamura⁵  Masayuki Ishihara⁵ 

[1] Department of Global Infectious Diseases and Tropical Medicine, National Defense Medical College, 3-2 Namiki, 359-8513, Tokorozawa, Saitama, Japan;Department of Oral and Maxillofacial Surgery, National Defense Medical College, 3-2 Namiki, 359-8513, Tokorozawa, Saitama, Japan;Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, 191-0065, Tokyo, Japan;Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, 191-0065, Tokyo, Japan;Research Institute, National Defense Medical College, 3-2 Namiki, 359-1324, Tokorozawa, Saitama, Japan;Research Institute, National Defense Medical College, 3-2 Namiki, 359-1324, Tokorozawa, Saitama, Japan;

关键词: Antimicrobial biomaterials;    Chitin nanofiber sheets;    Silver nanoparticles;    Wound dressings;    Anti-virus sheets;   

DOI  :  10.1186/s12951-014-0049-1

 received in 2014-08-18, accepted in 2014-11-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundChitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).Materials and methodsCNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.ResultsThe TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.ConclusionsOur results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

【 授权许可】

Unknown   
© Nguyen et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.