Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites

Journal of Nanobiotechnology

Takemi Matsui²  Yasushi Miyahira³  Takeshi Ono³  Shingo Nakamura⁴  Hidemi Hattori⁴  Jun Kinoda¹  Masayuki Ishihara⁴  Vinh Quang Nguyen⁴ 

[1] Department of Oral and Maxillofacial Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa 359-8513, Saitama, Japan;Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino 191-0065, Tokyo, Japan;Department of Global Infectious Diseases and Tropical Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa 359-8513, Saitama, Japan;Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa 359-1324, Saitama, Japan

关键词: Anti-virus sheets;    Wound dressings;    Silver nanoparticles;    Chitin nanofiber sheets;    Antimicrobial biomaterials;   

Others  :  1139310
DOI  :  10.1186/s12951-014-0049-1

 received in 2014-08-18, accepted in 2014-11-04,  发布年份 2014

【 摘 要 】

Background

Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods

CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results

The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions

Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

【 授权许可】

   
2014 Nguyen et al.; licensee BioMed Central Ltd.

【 图 表 】

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