High prevalence of Enterocytozoon bieneusi zoonotic genotype D in captive golden snub-nosed monkey (Rhinopithecus roxellanae) in zoos in China

BMC Veterinary Research

Research Article

Huili Zhu¹  Suhui Hu²  Yayun Wu²  Sumei Zhang²  Jianke Cao²  Changshen Ning²  Fuchang Yu²  Rongjun Wang²  Longxian Zhang²  Tongyi Li³  Jiantang Wang³ 

[1] College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, People’s Republic of China;College of Animal Science, Henan Institute of Science and Technology, 453003, Xinxiang, People’s Republic of China;College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, People’s Republic of China;International Joint Research Laboratory for Zoonotic Diseases of Henan, 450002, Zhengzhou, People’s Republic of China;Zhengzhou Zoo, 450000, Zhengzhou, People’s Republic of China;

关键词: Microsporidia;    Molecular characterization;    Phylogeny;    Nonhuman primates;   

DOI  :  10.1186/s12917-017-1084-6

 received in 2016-09-03, accepted in 2017-05-31,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundEnterocytozoon bieneusi is the dominant specie of microsporidia which can infect both anthroponotic and zoonotic species. The golden snub-nosed monkey is an endangered primate which can also infect by E. bieneusi. To date, few genetic data on E. bieneusi from golden snub-nosed monkeys has been published. Therefore, to clarify the prevalence and genotypes of E. bieneusi in captive golden snub-nosed monkeys is necessary to assess the potential for zoonotic transmission.ResultWe examined 160 golden snub-nosed monkeys from six zoos in four cities in China, using PCR and comparative sequence analysis of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi was 46.2% (74/160); while the prevalence was 26.7%, 69.1%, 69.4% and 33.3% in Shanghai Zoo, Shanghai Wild Animal Park, Tongling Zoo, and Taiyuan Zoo respectively (P = 0.006). A total of seven E. bieneusi genotypes were found that included four known (D, J, CHG1, and CHG14) and three new (CM19–CM 21) genotypes. The most common genotype was D (54/74, 73.0%), followed by J (14/74, 18.9%); other genotypes were restricted to one or two samples. Phylogenetic analysis revealed that genotype D belonged to the previously-characterized Group 1, with zoonotic potential; whereas genotypes J, CHG1, CHG14 and CM19–CM 21 clustered in the previously-characterized Group 2, the so-called cattle host specificity group.ConclusionsThe findings of high prevalence of zoonotic E. bieneusi genotypes D and J in golden snub-nosed monkeys suggest that golden snub-nosed monkeys may be the reservoir hosts for human microsporidiosis, and vice versa.

【 授权许可】

CC BY   
© The Author(s). 2017