Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes*

Colombia Médica

Sara María Robledo¹  Juan Fernando Alzate¹  Edwin Bairon Patiño¹  Antonio Jiménez-Ruiz¹  Miguel A Toro-Londoño¹ 

关键词: Leishmania panamensis;    Endonuclease G;    Molecular characterization;    Recombinant proteins;    Refolding;    Phylogeny;   

DOI  :  

学科分类：医学（综合）

来源: Universidad del Valle * Facultad de Salud / Universidad del Valle, Faculty of Health

【 摘 要 】

Objective:To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods:The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion, temperature, and pH preferences. Results:The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions:L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range.

【 授权许可】

Unknown