MicroRNAs associated with AGL6 and IAA9 function in tomato fruit set

BMC Research Notes

Research Note

Federica Pennisi¹  Nicola Vitulo¹  Tiziana Pandolfini¹  Barbara Molesini¹ 

[1] Department of Biotechnology, University of Verona, 37134, Verona, Italy;

关键词: MicroRNA;    RNA-Seq;    Fruit set;    Tomato;    RNA silencing;    Parthenocarpy;   

DOI  :  10.1186/s13104-023-06510-z

 received in 2023-01-31, accepted in 2023-09-15,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectiveFruit set is triggered after ovule fertilization, as a consequence of the downregulation of ovary growth repressors, such as the tomato transcription factors Auxin/indole-3-acetic acid 9 (IAA9) and Agamous-like 6 (AGL6). In a recent work, we developed a method to silence IAA9 and AGL6 in tomato ovaries using exogenous dsRNAs. We also produced small RNA libraries from IAA9- and AGL6-silenced ovaries to confirm the presence of siRNAs, derived from exogenous dsRNA, targeting IAA9 and AGL6. The objective of this work is to exploit these sRNA libraries to identify miRNAs differentially expressed in IAA9- and AGL6-silenced ovaries as compared with unpollinated control ovaries.ResultsWe identified by RNA sequencing 125 and 104 known and 509 and 516 novel miRNAs from reads mapped to mature or hairpin sequences, respectively. Of the known miRNAs, 7 and 45 were differentially expressed in IAA9- and AGL6-silenced ovaries compared to control ones, respectively. Six miRNAs were common to both datasets, suggesting their importance in the fruit set process. The expression pattern of two of these (miR393 and miR482e-5p) was verified by stem-loop qRT-PCR. The identified miRNAs represent a pool of regulatory sRNAs potentially involved in tomato fruit initiation.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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