Identification and expression profiling of microRNAs involved in the stigma exsertion under high-temperature stress in tomato

BMC Genomics

Research Article

Yi Zheng¹  Zhangjun Fei²  Changtian Pan³  Lifei Chen³  Xue Liu³  Dandan Yang³  Lei Ye³  Youwei Zhang³  Yan Wang³  Gang Lu⁴ 

[1] Boyce Thompson Institute, Cornell University, 14853, Ithaca, NY, USA;Boyce Thompson Institute, Cornell University, 14853, Ithaca, NY, USA;USDA Robert W. Holley Center for Agriculture and Health, 14853, Ithaca, NY, USA;Key Laboratory of Horticultural Plant Growth, Development and Biotechnology, Agricultural Ministry of China, Department of Horticulture, Zhejiang University, 310085, Hangzhou, China;Key Laboratory of Horticultural Plant Growth, Development and Biotechnology, Agricultural Ministry of China, Department of Horticulture, Zhejiang University, 310085, Hangzhou, China;Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, 310085, Hangzhou, China;

关键词: Tomato;    Heat stress;    MicroRNA;    Stamen;    Pistil;    High-throughput sequencing;   

DOI  :  10.1186/s12864-017-4238-9

 received in 2016-12-28, accepted in 2017-10-25,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAutogamy in cultivated tomato varieties is a derived trait from wild type tomato plants, which are mostly allogamous. However, environmental stresses can cause morphological defects in tomato flowers and hinder autogamy. Under elevated temperatures, tomato plants usually exhibit the phenotype of stigma exsertion, with severely hindered self-pollination and fruit setting, whereas the inherent mechanism of stigma exsertion have been hitherto unknown. Numerous small RNAs (sRNAs) have been shown to play significant roles in plant development and stress responses, however, none of them have been studied with respect to stamen and pistil development under high-temperature conditions. We investigated the associations between stigma exsertion and small RNAs using high-throughput sequencing technology and molecular biology approaches.ResultsSixteen sRNA libraries of Micro-Tom were constructed from plants stamen and pistil samples and sequenced after 2 d and 12 d of exposure to heat stress, respectively, from which a total of 110 known and 84 novel miRNAs were identified. Under heat stress conditions, 34 known and 35 novel miRNAs were differentially expressed in stamens, and 20 known and 10 novel miRNAs were differentially expressed in pistils. GO and KEGG pathway analysis showed that the predicted target genes of differentially expressed miRNAs were significantly enriched in metabolic pathways in both stamen and pistil libraries. Potential miRNA-target cleavage cascades that correlated with the regulation of stigma exsertion under heat stress conditions were found and validated through qRT-PCR and RLM-5′ RACE.ConclusionOverall, a global spectrum of known and novel miRNAs involved in tomato stigma exsertion and induced by high temperatures were identified using high-throughput sequencing and molecular biology approaches, laying a foundation for revealing the miRNA-mediated regulatory network involved in the development of tomato stamens and pistils under high-temperature conditions.

【 授权许可】

CC BY   
© The Author(s). 2017