Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity

BMC Genomics

Research Article

Ramanjulu Sunkar¹  Mahmoud W. Yaish²  Dekoum V. M. Assaha²  Himanshu V. Patankar²  Rashid Al-Yahyai³  Yun Zheng⁴ 

[1] Department of Biochemistry and Molecular Biology, Oklahoma State University, 74078, Stillwater, OK, USA;Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman;Department of Crop Science, College of Agriculture and Marine Sciences, Sultan Qaboos University, Muscat, Oman;Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China;

关键词: date palm;    differentially expressed genes;    DEGs;    leaves;    RNA-seq;    roots;    salinity;   

DOI  :  10.1186/s12864-017-3633-6

 received in 2016-07-14, accepted in 2017-03-16,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundDate palm, as one of the most important fruit crops in North African and West Asian countries including Oman, is facing serious growth problems due to salinity, arising from persistent use of saline water for irrigation. Although date palm is a relatively salt-tolerant plant species, its adaptive mechanisms to salt stress are largely unknown.ResultsIn order to get an insight into molecular mechanisms of salt tolerance, RNA was profiled in leaves and roots of date palm seedlings subjected to NaCl for 10 days. Under salt stress, photosynthetic parameters were differentially affected; all gas exchange parameters were decreased but the quantum yield of PSII was unaffected while non-photochemical quenching was increased. Analyses of gene expression profiles revealed 2630 and 4687 genes were differentially expressed in leaves and roots, respectively, under salt stress. Of these, 194 genes were identified as commonly responding in both the tissue sources. Gene ontology (GO) analysis in leaves revealed enrichment of transcripts involved in metabolic pathways including photosynthesis, sucrose and starch metabolism, and oxidative phosphorylation, while in roots genes involved in membrane transport, phenylpropanoid biosynthesis, purine, thiamine, and tryptophan metabolism, and casparian strip development were enriched. Differentially expressed genes (DEGs) common to both tissues included the auxin responsive gene, GH3, a putative potassium transporter 8 and vacuolar membrane proton pump.ConclusionsLeaf and root tissues respond differentially to salinity stress and this study has revealed genes and pathways that are associated with responses to elevated NaCl levels and thus may play important roles in salt tolerance providing a foundation for functional characterization of salt stress-responsive genes in the date palm.

【 授权许可】

CC BY   
© The Author(s). 2017