Suction assisted liposuction does not impair the regenerative potential of adipose derived stem cells

Journal of Translational Medicine

Research

Arndt F. Schilling¹  Hans-Günther Machens¹  David Atashroo²  Michael S. Hu²  Geoffrey C. Gurtner²  Michelle Lin²  Robert C. Rennert²  Zeshaan N. Maan²  Anna Luan²  Elizabeth A. Brett²  Alexander J. Whittam²  Natalie Ho²  Derrick C. Wan²  Michael T. Longaker³  Graham G. Walmsley³  Dominik Duscher⁴  Raphael Wenny⁵  Georg M. Huemer⁵  Manfred Schmidt⁵ 

[1] Department of Plastic Surgery and Hand Surgery, Technical University Munich, Munich, Germany;Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA;Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA;Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA;Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA;Section of Plastic, Aesthetic and Reconstructive Surgery, Johannes Kepler University, Linz, Austria;Department of Plastic Surgery and Hand Surgery, Technical University Munich, Munich, Germany;Section of Plastic, Aesthetic and Reconstructive Surgery, Johannes Kepler University, Linz, Austria;

关键词: Osteogenic Differentiation;    Adipogenic Differentiation;    Stromal Vascular Fraction;    Osteogenic Differentiation Medium;    Osteogenic Differentiation Potential;   

DOI  :  10.1186/s12967-016-0881-1

 received in 2016-02-09, accepted in 2016-04-27,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAdipose-derived stem cells (ASCs) have been identified as a population of multipotent cells with promising applications in tissue engineering and regenerative medicine. ASCs are abundant in fat tissue, which can be safely harvested through the minimally invasive procedure of liposuction. However, there exist a variety of different harvesting methods, with unclear impact on ASC regenerative potential. The aim of this study was thus to compare the functionality of ASCs derived from the common technique of suction-assisted lipoaspiration (SAL) versus resection.MethodsHuman adipose tissue was obtained from paired abdominoplasty and SAL samples from three female donors, and was processed to isolate the stromal vascular fraction. Fluorescence-activated cell sorting was used to determine ASC yield, and cell viability was assayed. Adipogenic and osteogenic differentiation capacity were assessed in vitro using phenotypic staining and quantification of gene expression. Finally, ASCs were applied in an in vivo model of tissue repair to evaluate their regenerative potential.ResultsSAL specimens provided significantly fewer ASCs when compared to excised fat tissue, however, with equivalent viability. SAL-derived ASCs demonstrated greater expression of the adipogenic markers FABP-4 and LPL, although this did not result in a difference in adipogenic differentiation. There were no differences detected in osteogenic differentiation capacity as measured by alkaline phosphatase, mineralization or osteogenic gene expression. Both SAL- and resection-derived ASCs enhanced significantly cutaneous healing and vascularization in vivo, with no significant difference between the two groups.ConclusionSAL provides viable ASCs with full capacity for multi-lineage differentiation and tissue regeneration, and is an effective method of obtaining ASCs for cell-based therapies.

【 授权许可】

CC BY   
© Duscher et al. 2016