Mechanical strain modulates age-related changes in the proliferation and differentiation of mouse adipose-derived stromal cells

BMC Cell Biology

Research Article

See-Chang Huang¹  Chun-Min Liu¹  Wen-Sheng Chiang¹  Hsiao-Chi Yu²  Tzu-Chin Wu²  Mei-Ru Chen²  Kurt M Lin³ 

[1] Biomedical Engineering Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan;Division of Medical Engineering Research, National Health Research Institutes, Zhunan Town, Miaoli, Taiwan;Division of Medical Engineering Research, National Health Research Institutes, Zhunan Town, Miaoli, Taiwan;Department of Biomedical Imaging and Radiological Sciences, National Yang Ming University, Taiwan;

关键词: Mechanical Loading;    Osteogenic Differentiation;    Mechanical Strain;    Adipogenic Differentiation;    Osteogenic Potential;   

DOI  :  10.1186/1471-2121-11-18

 received in 2009-07-13, accepted in 2010-03-10,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPrevious studies on the effects of aging in human and mouse mesenchymal stem cells suggest that a decline in the number and differentiation potential of stem cells may contribute to aging and aging-related diseases. In this report, we used stromal cells isolated from adipose tissue (ADSCs) of young (8-10 weeks), adult (5 months), and old (21 months) mice to test the hypothesis that mechanical loading modifies aging-related changes in the self-renewal and osteogenic and adipogenic differentiation potential of these cells.ResultsWe show that aging significantly reduced the proliferation and increased the adipogenesis of ADSCs, while the osteogenic potential is not significantly reduced by aging. Mechanical loading (10% cyclic stretching, 0.5 Hz, 48 h) increased the subsequent proliferation of ADSCs from mice of all ages. Although the number of osteogenic colonies with calcium deposition was increased in ADSCs subjected to pre-strain, it resulted from an increase in colony number rather than from an increase in osteogenic potential after strain. Pre-strain significantly reduced the number of oil droplets and the expression of adipogenic marker genes in adult and old ADSCs. Simultaneously subjecting ADSCs to mechanical loading and adipogenic induction resulted in a stronger inhibition of adipogenesis than that caused by pre-strain. The reduction of adipogenesis by mechanical strain was loading-magnitude dependent: loading with 2% strain only resulted in a partial inhibition, and loading with 0.5% strain could not inhibit adipogenesis in ADSCs.ConclusionsWe demonstrate that mechanical stretching counteracts the loss of self-renewal in aging ADSCs by enhancing their proliferation and, at the same time, reduces the heightened adipogenesis of old cells. These findings are important for the further study of stem cell control and treatment for a variety of aging related diseases.

【 授权许可】

Unknown   
© Huang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.