Characterization of the clonal profile of MRSA isolated in neonatal and pediatric intensive care units of a University Hospital

Annals of Clinical Microbiology and Antimicrobials

Research

Danilo Flªvio Moraes Riboli¹  Valéria Cataneli Pereira¹  Maria de Lourdes Ribeiro de Souza da Cunha¹ 

[1] Laboratory of Bacteriology, Department of Microbiology and Immunology, Institute of Biosciences, UNESP - Univ Estadual Paulista, CEP 18618-970, Botucatu,Sªo Paulo, Brazil;

关键词: Nosocomial infections;    NICU;    PICU;    MRSA;   

DOI  :  10.1186/s12941-014-0050-4

 received in 2014-05-14, accepted in 2014-10-17,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) are important pathogens in neonatal and pediatric intensive care units, which can cause severe infections in hospitalized children. Detection of the mec A gene and classification of the staphylococcal cassette chromosome mec (SCCmec) permit the characterization of MRSA strains isolated from infections caused by these microorganisms. In contrast, pulsed-field gel electrophoresis (PFGE) is used to type MRSA clones. This method is commonly used to analyze the epidemiology of bacteria causing nosocomial infections. The objective of this study was to detect and characterize MRSA isolated from clinical specimens of children hospitalized in the neonatal and pediatric intensive care units of the University Hospital of the Botucatu Medical School.MethodsA total of 119SS. aureus strains were isolated from clinical specimens and the mec A gene was detected by PCR. SCCmec was detected by multiplex PCR and the clonal profile was analyzed by PFGE.ResultsThe mec A gene was detected in 17.6% (21/119) of the isolates; 42.9% (9/21) of MRSA were characterized as SCCmec type III and 57.1% (12/21) as type IV. Analysis of the clonal profile of these strains revealed three distinct clones, with SCCmec type III being related to the Brazilian endemic clone and type IV to clones JCSC4469 and USA800.ConclusionsReplacement of clonal groups occurred in the neonatal and pediatric units over the period studied, a fact highlighting the importance of improving hygiene practices and control measures of nosocomial infections in these units.

【 授权许可】

CC BY   
© Pereira et al.; licensee BioMed Central Ltd. 2014