Chemopreventive effect of chalcone derivative, L2H17, in colon cancer development

BMC Cancer

Research Article

Minxiao Chen¹  Jianmin Zhou¹  Guang Liang¹  Shuping Hu¹  Yi Wang¹  Wenbo Chen¹  Shanmei Xu¹  Junguo Hui²  Jiansong Ji³ 

[1] Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, 325035, Wenzhou, China;Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, 325035, Wenzhou, China;Department of Radiology, the 5th Affiliated Hospital, Wenzhou Medical University, Lishui, Zhejiang, China;Department of Radiology, the 5th Affiliated Hospital, Wenzhou Medical University, Lishui, Zhejiang, China;

关键词: Colon cancer;    Chalcone derivatives;    Metastasis;    NF-κB;    Akt;   

DOI  :  10.1186/s12885-015-1901-x

 received in 2015-05-07, accepted in 2015-11-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundColon cancer is the third most commonly diagnosed cancer and the second leading cause of cancer mortality worldwide. Chalcone and its derivatives are reported to exhibit anti-cancer effects in several cancer cell lines, including colon cancer cells. In addition, chalcones have advantages such as poor interaction with DNA and low risk of mutagenesity. In our previous study, a group of chalcone derivatives were synthesized and exhibited strong anti-inflammatory activities. In this study, we evaluated the anti-cancer effects of the chalcone derivative, L2H17, in colon cancer cells.MethodsThe cytotoxicities of L2H17 on various colon cancer cell lines were investigated by MTT and clonogenic assay. Cell cycle and apoptosis analysis were performed to evaluate the molecular mechanism of L2H17-mediated inhibition of tumor growth. Also, scratch wound and matrigel invasion experiments were performed to estimate the cell migration and invasion after L2H17 treatment. Finally, we observed the anti-colon cancer effects of L2H17 in vivo.ResultsOur data show that compound L2H17 exhibited selective cytotoxic effect on colon cancer cells, via inducing G0/G1 cell cycle arrest and apoptosis in CT26.WT cells. Furthermore, L2H17 treatment decreased cell migration and invasion of CT26.WT cells. In addition, L2H17 possessed marked anti-tumor activity in vivo. The molecular mechanism of L2H17-mediated inhibition of tumor promotion and progression were function through inactivated NF-κB and Akt signaling pathways.ConclusionsAll these findings show that L2H17 might be a potential growth inhibitory chalcones derivative for colon cancer cells.

【 授权许可】

CC BY   
© Xu et al. 2015