NF-κB potentiates tumor growth by suppressing a novel target LPTS

Cell Communication and Signaling

Research

Yingzhe Liu¹  Wei Xiang¹  Kun Zhao¹  Shenglan Shang¹  Along Hou¹  Rui Wang¹  Xia Kang¹  Yue Ma¹  Huan Luo¹  Fengtian He¹  Dongbo Liu¹  Yuanyin Zhao¹  Hongming Miao¹  Rongchen Shi¹  Hongping Miao² 

[1] Department of Biochemistry and Molecular Biology, Third Military Medical University, 400038, Chongqing, China;Department of Neurosurgery, Southwest Hospital, Third Military Medical University, 400038, Chongqing, China;

关键词: NF-κB;    LPTS;    Promoter;    Cervical cancer;    Colon cancer;   

DOI  :  10.1186/s12964-017-0196-8

 received in 2017-06-22, accepted in 2017-10-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundChronic inflammation is causally linked to the carcinogenesis and progression of most solid tumors. LPTS is a well-identified tumor suppressor by inhibiting telomerase activity and cancer cell growth. However, whether and how LPTS is regulated by inflammation signaling is still incompletely elucidated.MethodsReal-time PCR and western blotting were used to determine the expression of p65 and LPTS. Reporter gene assay, electrophoretic mobility shift assay and chromatin immunoprecipitation were performed to decipher the regulatory mechanism between p65 and LPTS. Cell counting kit-8 assays and xenograt models were used to detect p65-LPTS-regulated cancer cell growth in vitro and in vivo, respectively.ResultsHere we for the first time demonstrated that NF-κB could inhibit LPTS expression in the mRNA and protein levels in multiple cancer cells (e.g. cervical cancer and colon cancer cells). Mechanistically, NF-κB p65 could bind to two consensus response elements locating at −1143/−1136 and −888/−881 in the promoter region of human LPTS gene according to EMSA and ChIP assays. Mutation of those two binding sites rescued p65-suppressed LPTS promoter activity. Functionally, NF-κB regulated LPTS-dependent cell growth of cervical and colon cancers in vitro and in xenograft models. In translation studies, we verified that increased p65 expression was associated with decreased LPTS level in multiple solid cancers.ConclusionsTaken together, we revealed that NF-κB p65 potentiated tumor growth via suppressing a novel target LPTS. Modulation of NF-κB-LPTS axis represented a potential strategy for treatment of those inflammation-associated malignancies.

【 授权许可】

CC BY   
© The Author(s). 2017