Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus

Reproductive Biology and Endocrinology

Research

Hajime Kitano¹  Michiya Matsuyama¹  Sethu Selvaraj¹  Mitsuo Nyuji¹  Hayato Adachi¹  Akihiko Yamaguchi¹  Hirofumi Ohga¹  Kensuke Kaneko¹  Akio Shimizu² 

[1] Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, 812-8581, Fukuoka, Japan;National Research Institute of Fisheries Science, Fisheries Research Agency, Kanazawa, 236-8648, Yokohama, Japan;

关键词: Chub mackerel;    Perciform;    Asynchronous;    FSH;    LH;    Steroidogenesis;    Oocyte maturation;   

DOI  :  10.1186/1477-7827-10-71

 received in 2011-12-08, accepted in 2012-08-30,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.MethodsNative FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.ResultsBoth cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.ConclusionsPresent results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.

【 授权许可】

Unknown   
© Ohga et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.