Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus

Reproductive Biology and Endocrinology

Michiya Matsuyama¹  Akihiko Yamaguchi¹  Hayato Adachi¹  Mitsuo Nyuji¹  Sethu Selvaraj¹  Hajime Kitano¹  Akio Shimizu²  Kensuke Kaneko¹  Hirofumi Ohga¹ 

[1] Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan;National Research Institute of Fisheries Science, Fisheries Research Agency, Kanazawa, Yokohama 236-8648, Japan

关键词: Oocyte maturation;    Steroidogenesis;    LH;    FSH;    Asynchronous;    Perciform;    Chub mackerel;   

Others  :  1150200
DOI  :  10.1186/1477-7827-10-71

 received in 2011-12-08, accepted in 2012-08-30,  发布年份 2012

【 摘 要 】

Background

The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.

Methods

Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.

Results

Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.

Conclusions

Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.

【 授权许可】

   
2012 Ohga et al.; licensee BioMed Central Ltd.

【 图 表 】

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