Facile aerobic construction of iron based ferromagnetic nanostructures by a novel microbial nanofactory isolated from tropical freshwater wetlands

Microbial Cell Factories

Research

Raha Abdul Rahim¹  Patricia Jayshree Jacob¹  Mas Jaffri Masarudin²  Mohd Zobir Hussein³ 

[1] Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Institute of Biosciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;

关键词: Iron oxide nanoparticles;    Ferromagnetic nanoparticles;    Aerobic bacteria;    Extracellular biosynthesis;    Melanin mediated;    Tropical freshwater ecosystems;   

DOI  :  10.1186/s12934-017-0789-3

 received in 2017-05-30, accepted in 2017-10-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIron based ferromagnetic nanoparticles (IONP) have found a wide range of application in microelectronics, chemotherapeutic cell targeting, and as contrast enhancers in MRI. As such, the design of well-defined monodisperse IONPs is crucial to ensure effectiveness in these applications. Although these nanostructures are currently manufactured using chemical and physical processes, these methods are not environmentally conducive and weigh heavily on energy and outlays. Certain microorganisms have the innate ability to reduce metallic ions in aqueous solution and generate nano-sized IONP’s with narrow size distribution. Harnessing this potential is a way forward in constructing microbial nanofactories, capable of churning out high yields of well-defined IONP’s with physico-chemical characteristics on par with the synthetically produced ones.ResultsIn this work, we report the molecular characterization of an actinomycetes, isolated from tropical freshwater wetlands sediments, that demonstrated rapid aerobic extracellular reduction of ferric ions to generate iron based nanoparticles. Characterization of these nanoparticles was carried out using Field Emission Scanning Electron Microscope with energy dispersive X-ray spectroscopy (FESEM–EDX), Field Emission Transmission Electron Microscope (FETEM), Ultraviolet–Visible (UV–Vis) Spectrophotometer, dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FTIR). This process was carried out at room temperature and humidity and under aerobic conditions and could be developed as an environmental friendly, cost effective bioprocess for the production of IONP’s.ConclusionWhile it is undeniable that iron reducing microorganisms confer a largely untapped resource as potent nanofactories, these bioprocesses are largely anaerobic and hampered by the low reaction rates, highly stringent microbial cultural conditions and polydispersed nanostructures. In this work, the novel isolate demonstrated rapid, aerobic reduction of ferric ions in its extracellular matrix, resulting in IONPs of relatively narrow size distribution which are easily extracted and purified without the need for convoluted procedures. It is therefore hoped that this isolate could be potentially developed as an effective nanofactory in the future.

【 授权许可】

CC BY   
© The Author(s) 2017