Cytological maps of lampbrush chromosomes of European water frogs (Pelophylax esculentuscomplex) from the Eastern Ukraine

BMC Genetics

Research Article

Glib Mazepa¹  Juriy Rosanov²  Spartak Litvinchuk²  Alla Krasikova³  Alsu Saifitdinova³  Dmitry Dedukh³  Dmitry Shabanov⁴  Leo Borkin⁵ 

[1] Department of Ecology and Genetic, Population Biology and Conservation Biology, Evolutionary Biology Centre, Uppsala University, EBC Norbyvägen 18 D, 75236, Uppsala, Sweden;V.N. Karazin Kharkiv National University, Svobody Sq. 4, 61022, Kharkiv, Ukraine;Institute of Cytology Russian Academy of Sciences, Tikhoretsky pr. 4, 194064, St.Petersburg, Russia;Saint-Petersburg State University, Oranienbaumskoie sch. 2, 198504, Stary Peterhof, Saint-Petersburg, Russia;V.N. Karazin Kharkiv National University, Svobody Sq. 4, 61022, Kharkiv, Ukraine;Zoological Institute, Russian Academy of Sciences, Universitetskaia nab.1, 199034, St. Petersburg, Russia;

关键词: Centromere;    Chromosome;    European water frog;    Hybridization;    Karyotype;    Non-coding RNA;    Nuclear body;    Oocyte;    Telomere;   

DOI  :  10.1186/1471-2156-14-26

 received in 2013-01-03, accepted in 2013-04-04,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundHybridogenesis (hemiclonal inheritance) is a kind of clonal reproduction in which hybrids between parental species are reproduced by crossing with one of the parental species. European water frogs (Pelophylax esculentus complex) represent an appropriate model for studying interspecies hybridization, processes of hemiclonal inheritance and polyploidization. P. esculentus complex consists of two parental species, P. ridibundus (the lake frog) and P. lessonae (the pool frog), and their hybridogenetic hybrid – P. esculentus (the edible frog). Parental and hybrid frogs can reproduce syntopically and form hemiclonal population systems. For studying mechanisms underlying the maintenance of water frog population systems it is required to characterize the karyotypes transmitted in gametes of parental and different hybrid animals of both sexes.ResultsIn order to obtain an instrument for characterization of oocyte karyotypes in hybrid female frogs, we constructed cytological maps of lampbrush chromosomes from oocytes of both parental species originating in Eastern Ukraine. We further identified certain molecular components of chromosomal marker structures and mapped coilin-rich spheres and granules, chromosome associated nucleoli and special loops accumulating splicing factors. We recorded the dissimilarities between P. ridibundus and P. lessonae lampbrush chromosomes in the length of orthologous chromosomes, number and location of marker structures and interstitial (TTAGGG)n-repeat sites as well as activity of nucleolus organizer. Satellite repeat RrS1 was mapped in centromere regions of lampbrush chromosomes of the both species. Additionally, we discovered transcripts of RrS1 repeat in oocytes of P. ridibundus and P. lessonae. Moreover, G-rich transcripts of telomere repeat were revealed in association with terminal regions of P. ridibundus and P. lessonae lampbrush chromosomes.ConclusionsThe constructed cytological maps of lampbrush chromosomes of P. ridibundus and P. lessonae provide basis to define the type of genome transmitted within individual oocytes of P. esculentus females with different ploidy and from various population systems.

【 授权许可】

Unknown   
© Dedukh et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.