Inhibition of Epstein-Barr virus reactivation by the flavonoid apigenin

Journal of Biomedical Science

Research

Ching-Hwa Tsai¹  Chung-Chun Wu²  Sheng-Ping Chou²  Sheng-Yen Huang²  Hui-Yu Hsu²  Yu-Jhen Cheng²  Jen-Yang Chen³  Chih-Yeu Fang⁴ 

[1] Department of Microbiology, College of Medicine National Health Research Institutes, National Taiwan University, No.35, Keyan Road, Zhunan Town, Miaoli County, Taipei, Taiwan;National Institute of Cancer Research, National Health Research Institutes, No.35, Keyan Road, Zhunan Miaoli, Miaoli County, Taiwan;National Institute of Cancer Research, National Health Research Institutes, No.35, Keyan Road, Zhunan Miaoli, Miaoli County, Taiwan;Department of Microbiology, College of Medicine National Health Research Institutes, National Taiwan University, No.35, Keyan Road, Zhunan Town, Miaoli County, Taipei, Taiwan;National Institute of Cancer Research, National Health Research Institutes, No.35, Keyan Road, Zhunan Miaoli, Miaoli County, Taiwan;Department of Pathology, Wan Fang Hospital, Taipei Medical University, 116, Taipei, Taiwan;

关键词: Epstein-Barr virus;    Apigenin;    Reactivation;    Nasopharyngeal carcinoma;   

DOI  :  10.1186/s12929-016-0313-9

 received in 2016-05-09, accepted in 2016-12-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundLytic reactivation of EBV has been reported to play an important role in human diseases, including NPC carcinogenesis. Inhibition of EBV reactivation is considered to be of great benefit in the treatment of virus-associated diseases. For this purpose, we screened for inhibitory compounds and found that apigenin, a flavonoid, seemed to have the ability to inhibit EBV reactivation.MethodsWe performed western blotting, immunofluorescence and luciferase analyses to determine whether apigenin has anti-EBV activity.ResultsApigenin inhibited expression of the EBV lytic proteins, Zta, Rta, EAD and DNase in epithelial and B cells. It also reduced the number of EBV-reactivating cells detectable by immunofluorescence analysis. In addition, apigenin has been found to reduce dramatically the production of EBV virions. Luciferase reporter analysis was performed to determine the mechanism by which apigenin inhibits EBV reactivation: apigenin suppressed the activity of the immediate-early (IE) gene Zta and Rta promoters, suggesting it can block initiation of the EBV lytic cycle.ConclusionTaken together, apigenin inhibits EBV reactivation by suppressing the promoter activities of two viral IE genes, suggesting apigenin is a potential dietary compound for prevention of EBV reactivation.

【 授权许可】

CC BY   
© The Author(s). 2017