Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus

BMC Infectious Diseases

Research Article

Ousmane Faye¹  Scott C. Weaver²  Amadou Alpha Sall³  Oumar Faye³  Ousmane Faye³  Alioune Gaye⁴  Mawlouth Diallo⁴  Ibrahima Dia⁴  Diawo Diallo⁴  Yamar Ba⁴  Cheikh Tidiane Diagne⁵ 

[1] Département de Biologie Animale, Laboratoire d’Écologie Vectorielle et Parasitaire, Université Cheikh Anta Diop, Dakar, Senegal;Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 77555–0610, Galveston, TX, USA;Department of Pathology and Microbiology & Immunology, University of Texas Medical Branch, 77555–0610, Galveston, TX, USA;Unité des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal;Unité d’Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal;Unité d’Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal;Département de Biologie Animale, Laboratoire d’Écologie Vectorielle et Parasitaire, Université Cheikh Anta Diop, Dakar, Senegal;

关键词: Aedes;    Arboviruses;    Oral infection;    Senegal;    Vector competence;    West Africa;    Zika virus;   

DOI  :  10.1186/s12879-015-1231-2

 received in 2015-04-24, accepted in 2015-10-19,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundZika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence.MethodsWe assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5 % Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates.ResultsAll the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50 % of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva.ConclusionAll the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal.

【 授权许可】

CC BY   
© Diagne et al. 2015