Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus

BMC Infectious Diseases

Mawlouth Diallo²  Amadou Alpha Sall⁴  Scott C. Weaver³  Ousmane Faye¹  Ibrahima Dia²  Alioune Gaye²  Ousmane Faye⁴  Yamar Ba²  Oumar Faye⁴  Diawo Diallo²  Cheikh Tidiane Diagne¹ 

[1] Département de Biologie Animale, Laboratoire d’Écologie Vectorielle et Parasitaire, Université Cheikh Anta Diop, Dakar, Senegal;Unité d’Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, Dakar, BP 220,, Senegal;Department of Pathology and Microbiology & Immunology, University of Texas Medical Branch, Galveston 77555–0610, TX, USA;Unité des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, 36 Avenue Pasteur, Dakar, BP 220,, Senegal

关键词: Zika virus;    West Africa;    Vector competence;    Senegal;    Oral infection;    Arboviruses;    Aedes;   

Others  :  1231030
DOI  :  10.1186/s12879-015-1231-2

 received in 2015-04-24, accepted in 2015-10-19,  发布年份 2015

【 摘 要 】

Background

Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence.

Methods

We assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5 % Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates.

Results

All the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50 % of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva.

Conclusion

All the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal.

【 授权许可】

   
2015 Diagne et al.

【 图 表 】

Fig. 1.

Fig. 1.

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