Distinct transcriptional profiles of ozone stress in soybean (Glycine max) flowers and pods

BMC Plant Biology

Research Article

Courtney P Leisner¹  Ray Ming¹  Elizabeth A Ainsworth² 

[1] Department of Plant Biology, University of Illinois, Urbana-Champaign, 61801, Urbana, IL, USA;Department of Plant Biology, University of Illinois, Urbana-Champaign, 61801, Urbana, IL, USA;USDA ARS Global Change and Photosynthesis Research Unit, 1201 W. Gregory Drive, 61801, Urbana, IL, USA;

关键词: Oxidative stress;    Glycine max;    RNA-Sequencing;    Matrix metalloproteinases;    Cell wall modification;   

DOI  :  10.1186/s12870-014-0335-y

 received in 2014-07-16, accepted in 2014-11-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundTropospheric ozone (O3) is a secondary air pollutant and anthropogenic greenhouse gas. Concentrations of tropospheric O3 ([O3] have more than doubled since the Industrial Revolution, and are high enough to damage plant productivity. Soybean (Glycine max L. Merr.) is the world’s most important legume crop and is sensitive to O3. Current ground-level [O3] are estimated to reduce global soybean yields by 6% to 16%. In order to understand transcriptional mechanisms of yield loss in soybean, we examined the transcriptome of soybean flower and pod tissues exposed to elevated [O3] using RNA-Sequencing.ResultsElevated [O3] elicited a strong transcriptional response in flower and pod tissues, with increased expression of genes involved in signaling in both tissues. Flower tissues also responded to elevated [O3] by increasing expression of genes encoding matrix metalloproteinases (MMPs). MMPs are zinc- and calcium-dependent endopeptidases that have roles in programmed cell death, senescence and stress response in plants. Pod tissues responded to elevated [O3] by increasing expression of xyloglucan endotransglucosylase/hydrolase genes, which may be involved with increased pod dehiscence in elevated [O3].ConclusionsThis study established that gene expression in reproductive tissues of soybean are impacted by elevated [O3], and flowers and pods have distinct transcriptomic responses to elevated [O3].

【 授权许可】

Unknown   
© Leisner et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.