Up-regulated S100 calcium binding protein A8 in Plasmodium-infected patients correlates with CD4+CD25+Foxp3 regulatory T cell generation

Malaria Journal

Research

Youngjoo Sohn¹  Yoon-Joong Kang²  Tong-Soo Kim³  Sangeun Lee⁴  Hyeong-Woo Lee⁴  Won-Ja Lee⁵  Jung-Yeon Kim⁶ 

[1] Department of Anatomy, College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 130-701, Seoul, Republic of Korea;Department of Biomedical Science, Jungwon University, 367-805, Goesan, Chungbuk, Republic of Korea;Department of Parasitology and Tropical Medicine, Inha Research Institute for Medical Sciences, Inha University School of Medicine, 400-712, Incheon, Republic of Korea;Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, 32610, Gainesville, FL, USA;Division of Arbovirus, Korea National Institute of Health, 363-951, Osong, Republic of Korea;Division of Malaria and Parasitic Disease, Korea National Institute of Health, 363-951, Osong, Republic of Korea;

关键词: Malaria;    Treg Cell;    Vivax Malaria;    Malaria Patient;    Indirect Fluorescent Antibody Test;   

DOI  :  10.1186/s12936-015-0855-4

 received in 2015-06-30, accepted in 2015-08-22,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe pro-inflammatory S100 calcium binding protein A8 (S100A8) is elevated in the serum of patients with Plasmodium falciparum malaria, but its function in Plasmodium vivax malaria is not yet clear. This function was investigated in P. vivax-infected patients in this study.MethodsThe level of S100A8 in the serum was measured with ELISA. Full amino acids of S100A8 were synthesized to verify the functions for maturation of immature dendritic cell (iDC) and evaluation of CD4+CD25+Foxp3+ regulatory T (Treg) generation by mature DC (mDC).ResultsA higher amount of S100A8 was detected in vivax-infected patients (141.2 ± 61.849 ng/ml, n = 40) compared with normal control group (48.1 ± 27.384 ng/ml, n = 40). The level of S100A8 did not coincide with that of anti-malarial antibody measured by indirect fluorescent antibody test (IFAT) using parasite-infected red blood cells as antigen. Programmed death-ligand 1 (PD-L1) was up-regulated on the surface of iDCs following treatment with synthetic S100A8, not with synthetic MSP-1, AMA-1 and CSP, as compared to the expression seen for non-treated iDCs. The addition of red blood cells of infected patients to iDCs also elevated their surface expression of CD86. However, the serum levels of S100A8 decreased with increase in parasitaemia. DCs matured by sera containing S100A8 generated Treg cells from naïve T cells. The ratio of Treg cells generated was inversely proportional to the concentration of S100A8 in sera.ConclusionsTreg cells suppress the activity of cytotoxic T cells, which kill malaria parasites; therefore, the up-regulation of S100A8 in malaria patients may contribute to pathogen immune escape or tolerance.

【 授权许可】

CC BY   
© Lee et al. 2015