Experimental detection of short regulatory motifs in eukaryotic proteins: tips for good practice as well as for bad

Cell Communication and Signaling

Review

Holger Dinkel¹  Francesca Diella¹  Toby J. Gibson¹  Kim Van Roey² 

[1] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D69117, Heidelberg, Germany;Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D69117, Heidelberg, Germany;Health Services Research Unit, Operational Direction Public Health and Surveillance, Scientific Institute of Public Health (WIV-ISP), 1050, Brussels, Belgium;

关键词: Linear motifs;    Bioinformatics;    Molecular switches;    Protein complexes;    Cell regulation;    Experimental design;   

DOI  :  10.1186/s12964-015-0121-y

 received in 2015-07-17, accepted in 2015-11-13,  发布年份 2015

来源: Springer

【 摘 要 】

It has become clear in outline though not yet in detail how cellular regulatory and signalling systems are constructed. The essential machines are protein complexes that effect regulatory decisions by undergoing internal changes of state. Subcomponents of these cellular complexes are assembled into molecular switches. Many of these switches employ one or more short peptide motifs as toggles that can move between one or more sites within the switch system, the simplest being on-off switches. Paradoxically, these motif modules (termed short linear motifs or SLiMs) are both hugely abundant but difficult to research. So despite the many successes in identifying short regulatory protein motifs, it is thought that only the “tip of the iceberg” has been exposed. Experimental and bioinformatic motif discovery remain challenging and error prone. The advice presented in this article is aimed at helping researchers to uncover genuine protein motifs, whilst avoiding the pitfalls that lead to reports of false discovery.

【 授权许可】

CC BY   
© Gibson et al. 2016